Survivin反义寡核苷酸对婴幼儿增生期血管瘤内皮细胞影响的实验研究-外科学专业论文.docxVIP

PAGE PAGE 10 3 MTT 实验结果显示：Survivin ASODN 对婴幼儿血管瘤内皮细胞的生 长抑制作用随浓度增大而增强。 4 倒置相差显微镜观察显示：实验组转染 ASODN 后的血管瘤内皮细胞 出现皱缩、变圆、脱落；大部分细胞体积缩小，贴壁细胞数量减少，脱壁悬浮细 胞增多，生长缓慢。其中 ASODN 浓度为 600nmol/L、作用 72h 时此种改变最显著。 5 HE 染 色 显 示 ： 实 验 组 转 染 ASODN 后 的 血 管 瘤 内 皮 细 胞 多数变圆、变小，细胞核固缩、碎裂，核被染成深蓝色或蓝黑色；细胞膜皱褶、 卷曲，符合细胞凋亡征象。各对照组细胞仍保持原有的生长形状，细胞核规整， 染成均一蓝色，胞浆呈淡红色。 研究结论：1、Survivin ASODN 体外转染后可明显下调婴幼儿增生期血管瘤内皮细 胞 Survivin 蛋白、Survivin mRNA 表达水平，并可以激活 Caspase-3。 2、Survivin ASODN 可以抑制血管瘤内皮细胞增殖，可能通过激活 Caspase-3 诱导血管瘤内皮细胞凋亡。 3、Survivin 基因可能是控制婴幼儿血管瘤内皮细胞增殖或凋亡的关键 基因之一。 关 键 词：婴幼儿血管瘤； 内皮细胞； Survivin ASODN； 凋亡； 脂质体； Caspase-3 Effect of antisense oligonucleotide of Survivin on proliferative infantile hemangioma endothelial cells Graduate: LI Pan-deng Supervisor: ZHANG li Abstract Hemangioma is the most common tumor of infancy and childhood，occuring in 10% to 12%. These lesions are three to five times more common in girls than in boys. Hemangiomas demonstrate a characteristic proliferation phase, which lasts an average of 6 to 12 months.This is followed by a slow period of involution, which generally requires several or more years for completion. Between 7-8 years old 90% of hemangiomas come into spontaneous regression. So far little is understood about the pathogenesis of infanti1e hemangiomas. Many studies have revealed that the proliferation and involution phases are due to unbalance between proliferation and apoptosis of vascular endothelial cell. But so far the mechanism of proliferation and apoptosis is known very little. Survivin is the strongest inhibitor of apoptosis which is newly discovered. Some studies and preliminary study of this issue found that survivin protein in proliferative phase hemangioma was highly expressed and located in endothelial cells; Otherwise survivin protein in the involution phase of hemangioma was low or even no expressed. And possibly survivin interfered with endothelial cell apoptosis by inhibiting caspase-3 activity and could promote proliferation of endothelial cell. Interf