XJ高致病性禽流感流行病学调查-预防兽医专业论文.docxVIP

Ⅰ Ⅰ 摘 要 本研究旨在对 XJ 部分地区高致病性禽流感采用血清学和分子流行病学 调查的方法，对 XJ 高致病性禽流感流行、发生、分布规律等特点进行了初 步分析和研究.通过对临床上怀疑为高致病性禽流感未免疫禽和已经过免 疫的禽群 H5 亚型免疫抗体水平的监测，结果表明 XJ 地区免疫抗体合格率较 高的通常为种禽场，中小规模的商品禽场、散养禽免疫抗体合格率较低， 属高风险禽群。 首先应用 M 基因检测引物，通过 RT-PCR，筛选出禽流感阳性样品； 并 且再次采用 RT-PCR 技术对阳性样品进行 HA 基因片段的扩增，将 HA 阳性的 PCR 产物克隆到 pMD18-T 载体上，用碱裂解法小量提取质粒并送基因公司 进行测序。通过 GeneBank 中的 HA 序列进行氨基酸的同源性进化树分析， 结果发现：扩增的 9 份阳性样品序列之间同源性达到了 93.1 %～99.3%，说 明它们属于同一病毒群；9 份阳性样品与国内 12 株 H5N1 型的同源性达到了 91.7～98.6%，据此可以推断这 9 份阳性样品属于 H5 亚型。 关键词：禽流感病毒；HA 基因；RT-PCR；抗体水平检测；流行病学监测 Ⅱ Ⅱ Abstract H5N1 avian influenza (AI) poses big threat to public health, Our goal of the present study is to investigate the epidemic and genesis and disposition of Highly Pathogenic Avian Influenza in some area of Uygur Autonomous Region. We take of serology and molecular epidemiology investigation to perform this. The H5 subtype antibody level of vaccinated poultry and none vaccinated poultry which are suspected to be infected by AIV were been tested, including poultry raised in high density and the place which had been infected by AIV. The results show that, the breeding bird rais ed farms are mostly eligible in the antibody test, while middle to small-scale commercial raising farm and disseminated raised poultry are in high risk in XingJiang province. We previously design a series of primer of M gene, and then detected our sample by RT-PCR. The positive samples were collected. Then we obtain the HA gene by the same way, The amplified HA fragment was cloned into the plasmid pMD18-T and sequenced and blast with GeneBank, Sequence blast results revealed that the homology of our 9 positive samples are 93.1 %～ 99.3%, which shows they are in the same virus group. Compared with the 12 domestic H5N1 strain, the homology are about 91.7～98.6 %. The results confirmed the 9 samples belonged to H5 subtype. Key words: Avian influenza virus; HA Gene; RT-PCR; The immune antibody level detection; Epidemiological surveillance. 40 40 XJ 高致病性禽流感流行病学调查 英文缩写词 缩写名称 英文全称 中文名称