布拉迪酵母菌对菌群失调肉鸭肠道酶修复作用.docVIP

布拉迪酵母菌对菌群失调肉鸭肠道酶修复作用 　　摘要：研究了布拉迪酵母菌（Saccharomyces boulardii）对肠道菌群失调樱桃谷肉鸭肠道酶的修复作用。将25只樱桃谷肉鸭随机分成正常对照组（5只）、抗生素组（20只）。建立樱桃谷肉鸭菌群失调模型，菌群失调模型建立后，抗生素组分为4组，每组5只：即氨苄青霉素组，布拉迪酵母菌低剂量组（试验Ⅰ组）、中剂量组（试验Ⅱ组）、高剂量组（试验Ⅲ组），其中，氨苄青霉素组立即屠宰取样并测定相关指标，各剂量布拉迪酵母菌组按每天200 mL/只饲喂各剂量酵母菌菌悬液，正常对照组自由饮水，各组连续饮用15 d。观察各组肠黏膜酶活性和基因表达的变化。结果表明，布拉迪酵母菌具有调节和增强双糖酶的活性、营养物质的吸收能力，对肉鸭黏膜蛋白质含量无显著影响。 　　关键词：布拉迪酵母菌（Saccharomyces boulardii）；菌群失调；黏膜酶活；基因表达 　　中图分类号：S834 文献标识码：A 文章编号：0439-8114（2015）11-2690-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.11.035 　　Effect of Saccharomyces boulardii on Intestinal Enzyme Activity of 　　Meat Ducks with Intestinal Dysbacteriosis 　　WANG Kun，MA Zhi-min，CHANG Chao，WANG Xue-dong，WU Jin-e 　　（College of Food Science and Engineering， Wuhan Polytechnic University， Wuhan 430023， China） 　　Abstract：The experiment aimed at studying the repair effect on intestinal dysbacteriosis ducks by Saccharomyces boulardii. 25 Cherry valley ducks were randomly divided to 5 groups such as normal control group， four test groups which need construct intestinal dysbacteriosis model first for 10 days. After the dysbacteriosis model constructed the four groups were treated with ampicillin and different doses of Saccharomyces boulardii 200 mL per duck for 15 d. The ampicillin group were slaughtered after treatment and tested correlative index， and the Saccharomyces boulardii groups were observed about intestinal enzyme activity and gene expression. The results showed that Saccharomyces boulardii could moderate and enhance the activity of disaccharidase and absorption of nutrients， and had no significant effect on the intestinal mucosa protein of meat duck. 　　Key words： Saccharomyces boulardii；dysbacteriosis； mucosa enzyme activity；gene expression 　　随着养殖业的发展，长期大量滥用抗生素导致畜禽肠道结构发生了改变，并且影响动物的免疫机能，应用微生态制剂代替兽药改善动物肠道结构一直是畜牧研究学者关注的焦点。 　　正常情况下，肠道菌群具有特定的定性、定位和定量结构，种群间处于一种有利于机体健康的动态平衡状态。但当这些正常的微生物菌群受到宿主生理状况及饮食、药物等的影响时，肠道正常菌群在种类、数量和比例上发生异常的变化，偏离正常的生理状态，转变为病理性组合状态，即菌群失调[1]，临床上以腹泻为明显症状[2]。肠道菌群紊乱广泛存在于动物生产实践和临床医学实践中，肠道菌群失调将直