应用逆转录病毒pLXSNEGFP示踪观察电针任脉对MCAO大鼠SVZ区NSC增殖影响.docVIP

应用逆转录病毒pLXSNEGFP示踪观察电针任脉对MCAO大鼠SVZ区NSC增殖影响 　　摘 要:目的:利用侧脑室注射pLXSN-EGFP在体示踪观察电针任脉对缺血侧SVZ区NSC增殖的情况,探讨电针任脉治疗缺血性脑损伤的作用机制。方法:将病毒上清注入MCAO大鼠侧脑室以标记新增殖细胞,并用Nestin免疫荧光染色法鉴定NSC,并计数不同时相缺血侧SVZ区NSC。结果:脑缺血后不同时相缺血侧SVZ区均有新增殖NSC,且电针任脉组有更明显的增加。结论:缺血可激发SVZ区NSC的增殖,电针任脉可起到明显促进作用,推论这种作用可能是是任脉的妊养和促进脑缺血后神经修复功能的重要机制。 　　关键词:反转录病毒;Nestin;电针任脉;神经干细胞 　　中图分类号:R245.97 文献标识码:A 文章编号:1673-7717(2010)01-0010-03 　　 　　Study on the Effects of Electroacupuncture at the Conception 　　Vessel on Proliferation of Neural Stem Cells in the Subventricular Zone by Retrovirus 　　Plasmid pLXSN-EGFP Tracer Technique in Brain Ischemia Rats 　　YANG Zhuo?xin1,YANG Fu?xia1,YU Hai?bo1,WANG Ling1 ,PI Min1,LUO Wen?shu1,MA Xiao?ming2 　　(1.Shenzhen Hospital of Chinese Medicine,Shenzhen 518033,Guangdong,China; 　　2.Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China) 　　Abstract:Objective:To observe the effects of electro-acupuncture(EA) at the Conception Vessel(CV) on the proliferation of neural stem cells by Retrovirus Plasmid pLXSN-EGFP Tracer Technique in Brain Ischemia Rats in the subventricular zone and to explore the mechanism of EA at the CV in treating ischemic brain injury.Methods: Each group was applied to intraventricular injection Retrovirus Plasmid pLXSN-mediated EGFP after MCAO to mark new proliferative cells and Nestin immunofluorescence staining was done to identify whether the cells were neural stem cells(NSCs)or not, and also he number of proliferating cell was observed in different time.Results: EGFP-positive and EGFP/ Nestin-positive were found in subventricular zone (svz)in different time after cerebral ischemia,on the 7th day remarkably increased(P0.01).The number of EGFP-positive and EGFP/ Nestin positive cells in EA group was more than that in model group in the same time (P0.01).Conclusion: The proliferation of NSCs can be promoted by EA at the CV after focal cerebral ischemia.It may be one of important mechanisms of which Conception Vessel has the function of nourishment a