微小RNA21和TGF―β1在正常皮肤和增生性瘢痕组织中表达研究.docVIP

微小RNA21和TGF―β1在正常皮肤和增生性瘢痕组织中表达研究 　　[摘要]目的：探讨微小RNA-21（miRNA-21）基因与转化生长因子-β1（transforming growth factor-β，TGF-β1）基因在正常皮?w组织和增生性瘢痕组织中的表达，并分析其相关性。方法：选取本院烧伤整形科收治的16例烧伤整形患者的增生性瘢痕组织及其临近的正常皮肤组织，提取组织中的总RNA，采用实时荧光定量PCR（realtime fluorescence quantitative PCR，RT-qPCR）技术分别检测增生性瘢痕组织和正常组织中miRNA-21与TGF-β1基因表达量，对检测结果进行分析比较，并行相关性分析。结果：增生性瘢痕组织中miRNA-21基因表达明显上调，差异具有统计学意义（P0.05），其表达水平是正常皮肤组织的2.18倍；增生性瘢痕中TGF-β1的表达量多于正常皮肤组织，差异具有统计学意义（P0.05），其表达水平是正常皮肤组织的2.31倍；增生性瘢痕组织中miRNA21基因表达量与TGF-β1基因表达量呈正相关（r=0.836，P0.01）。结论：增生性瘢痕组织中miRNA-21和TGF-β1基因表达异常，可能与瘢痕组织形成相关。 　　[关键词]增生性瘢痕；微小RNA21；TGF-β1 　　[中图分类号]R619+.6 [文献标志码]A [文章编号]1008-6455（2017）10-0068-03 　　Abstract： Objective To investigate the expression of microRNA-21 （miRNA-21） gene and transforming growth factor-β（TGF-β1） gene in normal skin and hypertrophic scar tissue， and to analyze its correlation. Methods Collected from 16 hypertrophic scar tissues and adjacent normal skin tissues formed by burn and plastic surgery department in our hospital， total RNA was extracted from normal tissues and Hypertrophic scar tissue. The expressions of miRNA-21 and TGF-β1 gene in hypertrophic scar tissue and normal tissue were detected by RT-qPCR. The detection results were analyzed and compared， and the correlation analysis was carried out. Results The expression of miRNA-21 gene in hypertrophic scar tissue was significantly up-regulated， the difference was statistically significant（P0.05）， its expression level was 2.18 times of normal skin tissue. The expression of TGF-β1 in hypertrophic scar was higher than that in normal skin tissue（P0.05）. The expression level of TGF-β1 was 2.31 times higher than that of normal skin tissue. There was a positive correlation between the expression of miRNA-21 gene and the expression of TGF-β1 gene in hypertrophic scar tissue（r=0.836， P0.01）. Conclusion The abnormal expression of miRNA-21 and TGF-β1 gene in hypertrophic scar tissue may be related to the formation of scar tissue. 　　Key words： hypertrophic scar； microRNA-21