慢性乙型肝炎患者中Ficolin―2与肝脏炎症相关性研究.docVIP

慢性乙型肝炎患者中Ficolin―2与肝脏炎症相关性研究 　　【摘要】 目的：研究HBV感染后慢性肝炎患者中纤维凝胶蛋白2（Ficolin-2）的表达及其与肝脏炎症进展之间的关系。方法：研究对象为101例HBV感染后慢性肝炎患者和120例健康对照者，采用酶联免疫吸附实验（enzyme-linked immunosorbent assay，ELISA）检测血浆Ficolin-2水平，全自动生化分析仪检测丙氨酸氨基转移酶（alanine aminotransferase，ALT）水平，实时荧光定量PCR技术（real-time quantitative polymerase chain reaction，real-time PCR）检测HBV DNA含量。结果：慢性肝炎患者血浆Ficolin-2水平明显高于健康对照组[（24.19±11.05）μg/ml vs （11.77±5.55）μg/ml]，差异有统计学意义（P0.01），ALT水平也明显高于健康对照组[（156.22±72.55）IU/L vs （20.58±8.87）IU/L]，差异有统计学意义（P0.01），线性回归分析表明血浆Ficolin-2水平与ALT水平呈正相关（R=0.813，R2=0.661，P0.01），而与HBV DNA含量未见明显相关性（R=0.105，R2=0.011，P=0.296）。结论：HBV感染后慢性肝炎患者血浆中Ficolin-2的表达与肝脏炎症程度密切相关，可以作为一个有效的辅助指标来评价肝脏炎症程度。 　　【关键词】 纤维凝胶蛋白2； 乙型肝炎病毒； 慢性肝炎； 丙氨酸转氨酶； HBV DNA 　　doi：10.14033/ki.cfmr.2018.8.002 文献标识码 A 文章编号 1674-6805（2018）08-0003-04 　　The Correclation between Ficolin-2 and Hepatic inflammation in Chronic Hepatitis Patients with HBV Infection/HU Min.//Chinese and Foreign Medical Research，2018，16（8）：3-6 　　【Abstract】 Objective：To investigate the possible relationships between expressions of Ficolin-2 and the progression of chronic HBV infection.Method：Ficolin-2 concentrations were measured by an enzyme-linked immunosorbent assay（ELISA） in the plasma of 101 chronic hepatitis patients with HBV infection and 120 healthy controls.Alanine aminotransferase（ALT） levels were detected using an automatic biochemical analyser.HBV DNA concentrations were measured using commercial realtime RT-PCR kits on an Applied Biosystems Prism 7500 seuqence detector.Result：Plasma levels of Ficolin-2 was （24.19±11.05） μg/ml in chronic hepatitis patients with HBV infection，it was higher than（11.77±5.55）μg/ml in healthy controls（P0.01），and the result of ALT was （156.22±72.55）IU/L in chronic hepatitis patients with HBV infection，it was higher than （20.58±8.87）IU/L in healthy controls（P0.01）.Ficolin-2 levels were positively related to the concentrations of ALT（R=0.813，R2=0.661，P0.01），and had no relattions with HBV DNA（R=0.105，R2=0.011，P=0.296）.Conclusion：Hig