【毕业论文】不同油脂对体外培养瘤胃微生物转氨酶和脱氢酶的影响.docVIP

PAGE l l 不同油脂对体外培养瘤胃微生物转氨酶和脱氢酶的影响 摘 要 本试验利用3头瘘管山羊提供瘤胃液进行体外培养，通过培养液转氨酶、脱氢酶等酶活指标的测定，考察花生油、菜油、玉米油和豆油等对微生物活力的影响。结果表明：乳酸脱氢酶、谷草转氨酶、谷丙转氨酶与对照组以及油脂试验组间都有显著差异（p0.05）。3种酶都以菜籽油最高、依次为豆油、玉米油、花生油等组，除花生油组与对照组差异不显著外（p0.05），其他油脂组都显著高于对照组（p0.05）。油脂组总脱氢酶与对照组之间有显著差异，添加油脂的试验组间也有显著差异（p0.05），其中以豆油组最高，显著高于对照组（p0.05）。总体来看，饱和程度不同的油脂对瘤胃微生物体外培养液转氨酶、脱氢酶酶活有明显而不同影响，其中以豆油的促进微生物活力效应最佳，玉米油较好。 关键词:油脂；瘤胃微生物；转氨酶；脱氢酶 Effects of Different Oils on Transaminase and Dehydrogenase of Ruminal Microorganisms in Vitro Animal Science 80601 Zhang xin Advisor Wang Mengzhi Abstract The objectives of this paper were to measure the effects of different oils on transaminase and dehydrogenase of ruminal microorganisms in vitro, using 3 Xuhuai White Goats with permanent cannulas. The treatments were, control group (no oil), peanut oil, rapeseed oil, corn oil, and soybean oil respectively. The results showed that, significant differences were found in LDH (lactate dehydrogenase), GOT (glutamic oxaloacetic transaminase), and GPT (Glutamic-Pyruvic Transaminase) among treatments (p0.05). All of 3 kind’s enzymes in culture medium was all highest in rapeseed oil, and then were soybean oil peanut oil, corn oil, and rapeseed oil orderly. Except for peanut oil, the other 3 oils higher than control group significantly (p0.05). The total dehydrogenase of experimental groups were higher than that of the control group (p0.05), and significant difference was also found in the total dehydrogenas between oil groups (p0.05), with soybean oil showing a highest value. It was therefore concluded that, different oils had different effects on transaminase and dehydrogenase of ruminal microorganisms in vitro, and soybean oil, and corn oil was much better than the others. Key words: Oil; Rumen Microorganisms; Transaminase; Dehydrogenase 第一部分 文献综述 反刍动物能够以单胃肠动物不能利用的粗饲料为食、并将之转化为优质的动物性蛋白质，而被誉为“生物转化器”。反刍动物的生产在我国现代畜牧生产体系中尤显重要，对畜牧业的可持续发展也有着重要的意义。而反刍动物“生物转化器”的美名得益于其瘤胃中的微生物和微生物在瘤胃中的正常发酵。相反其发酵的异常，常常降低纤维素饲料的降解率和利用率，也影响宿主可以利用的微生物蛋白量，进而影响宿主