银杏杂交子代药用质量指标的变异研究探究 .pdf

安徽农业科学,Journal of Anhui Agri .Sci .2007 ,35(25) :7878 - 7880 责任编辑 庆瑢 责任校对 俞洁 银杏杂交子代药用质量指标的变异研究 1 2 3 莫昭展 , 曹福亮, 符韵林 ( 1. 玉林师范学院, 广西玉林537000 ;2 . 南京林业大学森林资源与环境学院, 江苏南京210037 ;3 . 广西大学林学院, 广西南宁530004) 摘要 [ 目的] 为 了准确地选择黄酮、内酯含量高的银杏叶用家系。[ 方法] 以一年生控制授粉 的银杏杂交子代苗 为试材, 采 用高效液相 色谱 法测定杂交子代的黄酮、内酯含量, 通过方差分析研 究银杏杂交 子代药用质量指标的变异情 况。[ 结果] 家系间叶片总黄酮含量差 异极显著( F = 82 .99 , Pr 0 .000 1) , 平均值是13 .63 mg/ g , 变异 系数是20 .09 % ; 家系间叶片银杏总内酯含量差异极显著( F = 33 .48 , Pr 0 .000 1) , 平均值是6 .86 mg/ g , 变异 系数是25 .82 % ; 家系间单株生物量差异极显著( F = 11 .63 , Pr 0 .000 1) , 平均值是4 .81 g , 变异系数是 19 .45 % ; 家系间叶片单株有效经济产量有极显著差异( F = 14 .46 , Pr 0 .000 1) , 平均值是20 .25 mg , 变异 系数是34 .14 % 。通过聚类分析 初步筛选 出优 良的叶用家系8 个 。[ 结论] 生 态 因子和不 同种源、品种 、采叶 时间、树龄 、采叶部位、加 工方法影 响银 杏叶黄 酮和 内酯含 量 。 关键词 银杏; 杂交子代; 黄酮; 内酯 中图分类号 S664 .3 文献标识码 A 文章编号 0517 - 6611(2007) 25 - 07878 - 03 Research on Variation of Medicine Quality Indices of Ginkgo bil oba L . Crossing Generation MO Zhao-zhan et al ( Yulin Nor mal College , Yuli n , Guangxi 537000) Abstract [ Obj ective] The study ai med to choose Ginkgo bil oba L. family lines with high flavone and terpene lactones contents accurately . [ Method] With 1-year- old hybrid filial generation seedlings of gi nkgo being controlled pollination as test materials , the flavone and lactones contents of hybrid filial generation were deter mined by HPLC and the variation of medicine quality indices of ginkgo hybrid filial generation was studied through analysis of vari- ance . [ Result] The total flavone content in leaves had extre mely significant difference among family lines ( F = 82 .99 , Pr 0 .000 1) , with the average of 13 .63 mg/ g and variation coefficient of 20 .09 % . The total lactones content in leaves had extre mely significant difference among family lines ( F = 33 .48 , Pr 0 .000 1) , w