覆盖野生稻全基因组染色体片段置换系构建进展-中国农业科学.PDFVIP

中国农业科学 2013,46(24):5075-5080 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.24.001 覆盖野生稻全基因组染色体片段置换系构建进展 叶新福，杨德卫 （福建省农业科学院水稻研究所，福州 350018 ） 摘要：野生稻长期处于野生状态，经受了各种灾害和不良环境的自然选择，保存着栽培稻不具有的或已经消 失了的特异基因，抗逆性较强，是天然的基因库。染色体片段置换系是进行基因分析的理想材料。建立染色体片 段置换系群体，不仅可以挖掘新的基因资源，还可以实现植物分子育种从单个基因利用到基因组综合利用的跨越 发展。文中介绍了染色体片段代换系群体的特点和构建原理，综述了野生稻染色体片段代换系群体构建及应用的 研究进展，并对其研究方向进行了分析和展望, 以便为今后开发和利用野生稻资源提供理论依据。 关键词：野生稻；染色体片段置换系；构建 Progress in Research of Chromosome Segment Substitution Line Carrying Overlapping Chromosome Segments of the Whole Wild Rice Genome YE Xin-fu, YANG De-wei (Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 35001 8) Abstract : Wild rice has adapted to weather and unfavorable environments under natural selection. It has been well recognized as a natural gene bank that conserves a lot of specific genes presently not available for extinct in the cultivated rice. The chromosome segment substitution lines (CSSLs) were perfect materials for analysis of gene. Developing the CSSLs can not only search and use the new gene resource ，but also can achieve the development of span mode of plant molecular breeding from utilizing single gene to synthetically exploiting genome. In this paper, the principle of developing the CSSLs was described, the research progress in construction and application was reviewed, and the prospect was discussed. So that it could provide a base for utilization of wild rice resource. Key words: wild rice; chromosome segment substitution lines (CSSLs); construction 要分布在中国南方地区，如广西、广东、云南、海南、 0 引言