杏香兔耳风高效液相色谱光电二极管矩阵检测器法指纹图谱研究.docVIP

杏香兔耳风高效液相色谱光电二极管矩阵检测器法指纹图谱研究 　　[摘要] 目的 研究用高效液相色谱-光电二极管矩阵检测器法（HPLC-PDAD）建立杏香兔耳风指纹图谱的分析方法，为杏香兔耳风的质量鉴定提供依据。 方法 色谱条件为：色谱柱为Nucleosil C18柱（4.6 mm×250 mm，5 μm），以乙腈-缓冲溶液（乙酸-NaH2PO4，0.1 mol/L，pH=2.5）为流动相，梯度洗脱，流速1.0 mL/min，柱温30℃，检测波长356 nm。 结果 用上述方法确定了12批不同品种杏香兔耳风的11个共有峰，其相识度均大于0.91，精密度、稳定性、重复性试验、各共有峰相对保留时间和相对峰面积的RSD均小于2.5%，各色谱峰分离效果良好，符合指纹图谱要求。 结论 该方法精密度、稳定性和重复性均较好，为杏香兔耳风的质量控制标准提供了参考。 　　[关键词] 杏香兔耳风；高效液相色谱-光电二极管矩阵检测器法；指纹图谱；质量控制 　　[中图分类号] R284.1[文献标识码] B[文章编号] 1673-7210（2014）05（c）-0101-04 　　 　　Study on HPLC-PDAD Fingerprints of Ainsliaea fragrans Champ. 　　WU Haojie1 ZOU Shengqin2 　　1.Traditional Chinese Medicine Hospital of Fenghua City, Zhejiang Province, Fenghua 315500, China; 2.College of Chemistry and Bioengineering, Yichun University, Jiangxi Province, Yichun 336000, China 　　[Abstract] Objective To establish the analytical method of Ainsliaea fragrans Champ. fingerprint chromatography by HPLC-PDAD. Methods The chromatographic conditions were as follows: a Nucleosil C18 (4.6 mm×250 mm, 5 μm) with CH3CN-Buffer solution (CH3COOH-NaH2PO4, 0.1 mol/L, pH=2.5) as mobile phase in a gradient mode. The flow rate was 1.0 mL/min, and the column temperature was 30℃. The detecting wavelength was 356 nm. Results 11 common peaks were found in the fingerprint of 12 batches of Ainsliaea fragrans Champ. and the similarity of 12 samples were more than 0.91. The established HPLC fingerprint chromatograms had desirable accuracy, repeatability and stability. Conclusion This method is accurate and steady with a good repeatability and provides a reference for quality control of Ainsliaea fragrans Champ.. 　　[Key words] Ainsliaea fragrans Champ.; HPLC-PDAD; Fingerprint; Quality control 　　杏香兔耳风为菊科兔耳风属植物杏香兔耳风Ainsliaea fragrans Champ.的干燥全草，别名白走马胎、金边兔耳草、一枝香等，天然或以人工栽培方式分布于长江以南各省区，收载于《卫生部药品标准》，具有清热解毒、舒筋活血、发表祛风等功效，主治妇女崩漏、痈疽肿毒、毒蛇咬伤[1-2]。临床上用于治疗肺结核咯血、跌打损伤、无名肿毒、慢性宫颈炎[3-4]。杏香兔耳风主要含有酚酸类、黄酮类、三萜类、倍半萜内酯、倍半萜苷等化学成分[5]。酚酸类化合物是其主要成分，其中绿原酸、3，4-二咖啡酰基奎宁酸、3，5-二咖啡酰基奎宁酸和4，5-二咖啡酰基奎宁酸等化合物含有量较高[6]；黄酮类成分主要有槲皮素、木犀草素、芹