正己烷对大鼠血热休克蛋白70和总抗氧化能力影响.docVIP

正己烷对大鼠血热休克蛋白70和总抗氧化能力影响 　　【中图分类号】R392.12 【文献标识码】A 【文章编号】1672－3783(2012)06－0033－01 　　【摘要】目的 研究正己烷(n-HeXane)对大鼠血中热休克蛋白(HSP70)和总抗氧化能力(T-AOC)的影响。方法 40只雄性SpragueDawley(SD)大鼠随机分成4组，即低、中、高剂量染毒组(150 mg/kg、300 mg/kg、600 mg/kg)和阴性对照组，每组10只，经腹腔注射染毒后，观察7天，用酶联免疫吸附法和试剂盒分别检测大鼠血中HSP70和T-AOC的含量。结果 各剂量组的一般情况与对照组比较均无异常；HSP70随染毒剂量增加而上升，T-AOC随染毒剂量增加而降低，与对照组差异明显(P 　　【关键词】正己烷;热休克蛋白70;总抗氧化能力 　　Effect of n- hexane on heat shock protein 70 and total antioxidant capacity in rats 　　【Abstract】 Objective To study the effect of n-hexane on heat shock protein 70 (HSP70) and total antioxidant capacity (T-AOC) in rats. Method 40 SD male rats were randomly divided into four groups. n-hexane was administered at doses of 0 mg/kg (the negative control group), 150mg/kg, 300mg/kg, 600mg/kg by intraperito-neal injection. All rats were observed for 7 days. Then all rats were sacrificed to obtain serum. The serum level of Hsp70 and T-AOC were determined. Result There were no significant differences between general situations of control group and exposure groups. Compared with the negative control group, exposure dose rising, content of Hsp70 increased, activity of T-AOC decreased among exposure groups presented a significant dose-effect relationship and significance of difference。(P 0.05). Conclusion The poisoning of n-hexane may be dynamic process. In the early event in the toxicity of n-hexane, the lipid peroxidation injury was caused, but Hsp70 and T-AOC have a protection effect. Therefore, Hsp70 and T-AOC can be used as early diagnosis indicators of n-hexane poisoning. 　　【Key words】 n-Hexane; heat shock protein 70 (Hsp70)；total antioxidant capacity (T-AOC) 　　正己烷是直链烷烃类化学毒物，具有高挥发性、高脂溶性和高蓄积作用特点，因其工业用途广和刺激性不大，易致职业人群中毒，严重威胁职业人群的生命安全，职业危害尤为突出，引起学者广泛关注。基于做好职业性正己烷中毒早期预防的考虑，寻找早期诊断生物指标尤为关键，本研究通过建立急性中毒动物模型，观察一般情况和检测血液中HSP70表达和T-AOC含量水平，探讨正己烷对机体早期毒作用与HSP70和T-AOC的生物学意义。 　　1 材料与方法 　　1.1.1 主要仪器与试剂：正己烷(国产分析纯)，T-AOC检测试剂盒购买于南京建成生物工程研究所，HP70检验试剂盒由厦门慧嘉生物科技有限公司提供，2100型分光光度计(尤尼柯仪器有限公司)。 　　1.1.2 动物：健康雄性Sprague-Dawley(SD)大鼠4