生物有机肥和EM菌剂对菜园连作土壤微生物影响.docVIP

生物有机肥和EM菌剂对菜园连作土壤微生物影响 　　摘 要 采用盆栽试验，连续种植3批菜心，研究生物有机肥和EM菌剂对连作9 a和6 a的菜园土壤微生物群落结构和功能多样性的影响。结果表明：与化肥处理相比，施用生物有机肥能够提高连作土壤中细菌、放线菌、丛枝菌根真菌和革兰氏阴性细菌（G-）的相对含量及G-/G+（革兰氏阴性细菌/革兰氏阳性细菌）比值，并降低土壤真菌的相对含量；随着种植批次的增加，土壤中G-、G+含量降低，G-/G+比值上升，生物有机肥和EM菌剂配施化肥处理G-含量普遍高于化肥处理；与化肥处理相比，施用生物有机肥土壤微生物AWCD值、Simpson指数、McIntosh指数均提高，土壤微生物活性、多样性和均一度提高。 　　关键词 生物有机肥 ；EM菌剂 ；土壤微生物 ；连作障碍 　　中图分类号 S963.91 文献标识码 A Doi：10.12008/j.issn.1009-2196.2017.04.013 　　Effects of Bio-organic Fertilizer and EM Microbial Agent on Microbes 　　of Continuous Cropping Soil in Vegetables Garden 　　CHEN Wen LIU Xiao SUN Guangwen LIU Houcheng ZHU Mengyu CHEN Riyuan 　　（College of Horticulture， South China Agricultural University， Guangzhou， Guangdong 510642） 　　Abstract Vegetable Choi Sum was pot tried for three rounds of cropping to observe the effects of bio-organic fertilizer and EM microbial agent on microbial community structure and functional diversity of the soil in the vegetable garden for 9 and 6 years of continuous cropping. The results showed that the bio-organic fertilizer increased the relative contents of bacteria， actinomycetes， arbuscular mycorrhizal fungi and gram-negative bacteria， and the gram-negative bacteria/gram-positive bacteria ratio of continuous cropping soil， but reduced the relative content of fungi， as compared to the chemical fertilizer. With the more croppings the bio-fertilizer reduced the relative contents of gram-negative bacteria， gram-positive bacteria， increased the gram-negative bacteria/ gram-positive bacteria ratio. The bio-organic fertilizer and the EM agents +chemical fertilizer treatment generally had a higher relative content of gram-negative bacteria than the chemical fertilizer treatment. Comparing with chemical fertilizer， the bio-fertilizer treatment had a higher average well color development （AWCD）， Simpson index and McIntosh index of soil microbes as well as soil microbial activity， diversity and uniformity than the chemical treatment. 　　Keywords bio-organic fertilizer ； EM