纳米银抗菌凝胶对大鼠深Ⅱ度烫伤创面愈合及巨噬细胞数量和微血管密度影响.docVIP

纳米银抗菌凝胶对大鼠深Ⅱ度烫伤创面愈合及巨噬细胞数量和微血管密度影响 　　[摘要]目的：?^察纳米银抗菌凝胶联合重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）对大鼠深Ⅱ度烫伤创面愈合、巨噬细胞数量以及微血管再生的影响。方法：选取健康SD大鼠114只（6只作为非烫伤正常对照组，不作任何处理），剩余SD大鼠按照随机数字法分为3组，rhGM-CSF组、联合组和模型组，每组36只。制作深Ⅱ度烫伤创面模型，rhGM-CSF组大鼠创面涂抹rhGM-CSF，联合组应用纳米银抗菌凝胶和rhGM-CSF涂抹，模型组不作任何处理。比较各组创面愈合率，创面巨噬细胞数量以及微血管密度数量。结果：同一时间点，rhGM-CSF组和联合组愈合率均高于模型组，且联合组愈合率显著大于rhGM-CSF组，差异有统计学意义（P0.05）；联合组第3、11、14天创面浸润巨噬细胞数量均少于模型组和rhGM-CSF组（P0.05）；第3、7、11天，联合组微血管数量显著高于模型组和rhGM-CSF组，差异有统计学意义（P0.05）。结论：纳米银抗菌凝胶联合rhGM-CSF治疗深Ⅱ度烫伤效果确切，可以通过影响烫伤创面巨噬细胞数量和微血管生成促进烫伤创面愈合。 　　[关键词]纳米银抗菌凝胶；重组人粒细胞巨噬细胞集落刺激因子；烫伤；巨噬细胞；微血管 　　[中图分类号]R644 [文献标志码]A [文章编号]1008-6455（2018）03-0104-04 　　Effect of Nano Silver Antibacterial Gel on the Deep Ⅱ Degrees of Rat Scalded Wound Healing and the Number of Macrophages， the Microvascular Density 　　ZHANG Zi-hao 1，GAO Su-yue2，CUI Wen-hao3 　　（1.Chinese PLA General Hospital，Beijing 100000，China；2.the Second Hospital Affiliated to Suzhou University，Suzhou 215004，Jiangsu，China；3.the First Peoples Hospital of Jinzhong，Jinzhong 030600，Shanxi，China） 　　Abstract： Objective To observe the effect of nano silver antibacterial gel joint rhGM-CSF on the deep Ⅱ degrees of rat scalded wound healing and the number of macrophages， the microvascular density. Methods A total of 114 healthy SD rats （6 rats served as non scald normal control group， without any treatment） were selected， the remaining SD rats were randomly divided into 3 groups， the rhGM-CSF group， the combination group and the model group， 36 rats in each group. The deep second degree scald wound model was made， the rhGM-CSF group was treated with rhGM-CSF， the combination group was treated with nano silver antibacterial gel and rhGM-CSF， and no treatment was given to rats of the model group. The rate of wound healing， the number of macrophages and the number of microvessel density in each group were compared. Results At the same time， the healing rate of the rhGM-CSF group and the combination group were higher than those of the model group， and the comb