半滑舌鳎、牙鲆微卫星遗传连锁图谱的构建-水产养殖学专业论文.docxVIP

III Construction of Microsatellite Genetic Linkage Maps for Half-smooth Tongue Sole and Japanese Flounder ABSTRACT Half-smooth tongue sole (Cynoglossus semilaevis) and Japanese flounder (Paralichthys olivaceus) are important cultured marine fish species in China. They have been the most popular fish species together with barfin flounder (Verasper moseri), Spotted halibut (Verasper variegatus) and turbot (Scophthalmus maximus) in the northern China. Half-smooth tongue sole (Cynoglossus semilaevis) is a commercially valuable flatfish that is widely distributed in Chinese coastal waters. Due to its commercial value, easy domestication and natural resource depletion, half-smooth tongue sole has been selected as a promising species for aquaculture. It has been one of the most popular marine species used in aquaculture in China. Japanese flounder (Paralichthys olivaceus) is a marine fish which is economically important as a food, and has been widely cultured in Asian countries such as China, Japan and Korea. It is mostly distributed along the coast of China, where it has been cultured for approximately 20 years due to its favourable traits such a fast growth rate, good adaptability to temperature and disease resistance in a variety of cultivation conditions. Recently, the number of wild individuals of the fish species is little, and the fisheries resource is rather critical. Meanwhile, the deterioration of mariculture environment cause the frequency of fish diseases, which results in increasing and appreciable economic damage, and also has adverse effect on the healthy development of aquaculture. The traditional methods of genetic improvement of quantitative traits have relied mainly on phenotype and pedigree information, which are easily influenced by environmental factors. It is generally accepted that marker-assisted selection (MAS) could accelerate genetic improvement in a relatively short period, especially when the target characteristics are disease-related