包被γ-氨基丁酸对奶牛热应激的防治效果及机理的研究-临床兽医学专业论文.docxVIP

 PAGE 6 Abstract This study was conducted to evaluate the effects of coated gamma-aminobutyric acid (GABA) in dariy cow under heat stress,and investigate on the related mechanism by immunity、endocrine and antioxidation. Twenty Holsteins lactating cows, offered the same basal diet, were blocked by days in milk, milk yield and parity and then randomly allocated toⅠof Ⅳ dietary treatments: negative control (group Ⅰ ), groups Ⅱ 、 Ⅲ 、 Ⅳ of coated GABA supplementation (5 g/d、10g/d and 15g/d),the cows were raised including 7 days of pretreating and 30 days for test.The milk yield was monitored by computer, Rectal temperature and respiratory rate were measured at the start of the study and after 14 and 29 d.The lactose、milk fat、lactoprotein and total milk solid were detected by Milk composition analyzer. The serum index were measured as follows: the concentration of GABA;the levels of CRH, ACTH and Cor; the concentration of potassium,sodium,calcium,phosphorus,chloride and HCO3－; the level of MDA, the activity of SOD and GSH-Px enzyme;the immunity of IL-2,IgG,IgM and IgA;the levels of T3,T4,GH and INS, the concentration of blood glucose and blood lipid.The results showed: (1).Rectal temperature and respiratory rate were lowerd by using coated GABA, On day-10 and 30, the rectal temperature of groups Ⅲ、Ⅳ were significantly lower than groupⅠ(P 0.01), the respiratory rate of group Ⅳ was lower than groupⅠ(P 0.05). (2).GABA concentration in the serum rose as GABA addition in feed increased, On day-15, the GABA concentration of group Ⅳ was higher than groupⅠ(P 0.05), On day-30, the GABA concentration of groups Ⅲ、Ⅳ was significantly higher than group Ⅰ(P 0.01). (3). Compared with groupⅠ, the addition of coated GABA decreased the hormone of HPA axis, the CRH level of groups Ⅲ、Ⅳ and Cor level of groups Ⅳ were lower (P 0.05), while the ACTH level of group Ⅳ was significantlydecreased (P 0.01). (4).The concentrations of sodium、calcium、phosphorus、chloride、