胰岛素血管紧张素II对人血管内皮细胞NFκB激活和血脂康干预影响.docVIP

胰岛素血管紧张素II对人血管内皮细胞NFκB激活和血脂康干预影响 　　［摘 要］ 目的 观察胰岛素、血管紧张素II(Ang II)对体外培养的 人脐静脉内皮细胞(Human umbilical vein endothelial cells, HUVECs)NF_κ B的激活及 血脂康干预后的影响。方法 体外培养人脐静脉内皮细胞株(ECV-304)，2 ～ 3代用于实验。细胞用0.5%胎牛血清培养液培养24小时后分成七组；正常对照组、胰岛素组 (100uI /ml)、Ang II组(5×10-8mol/ml)、胰岛素+Ang II组、胰岛素+血脂康组(150ug/ml) 、Ang II+血脂康组、胰岛素+Ang II+血脂康组。加血脂康的各组，血脂康预先孵育2h，再 加胰岛素或/和Ang II共同孵育。在4h时间点上，分别用免疫细胞化学方法测各组内皮细胞N F_κ Bp65的核易位阳性率，用免疫荧光和流式细胞仪检测其活性。结果 ①胰岛素、Ang II、胰岛素+Ang II刺激4h后，内皮细胞NF_κ Bp65的核易位阳性率(%)分别 为95.5±3、96.6±3、96.4±4，显著高于对照组(4.5±2)(P＜0.05)，血脂康几 乎完全抑制胰岛素、Ang II、胰岛素+Ang II所致的NF_κ Bp65核易位阳性率的增加(7.3 ±3、6.9±4、6.3±2)(P＜0.05)。②胰岛素、Ang II、胰岛素+Ang II刺激4h后， 内皮细胞NF_κ B活性分别为6.55±0.53、7.23±0.36、10.29±0.69，显著高于对照 组(3.26±0.37)(P＜0.05)；与单用胰岛素或Ang II比，胰岛素+Ang II使NF_κ B活 性增加更明显(P＜0.05)；血脂康能明显抑制NF_κ B的活性(4.44±0.49、3.98±0 .24、5.02±0.39)(P＜0.05)。结论 胰岛素、Ang II均能激活内 皮细胞NF_κ B，促进NF_κ Bp65的核易位，有致动脉粥样硬化(AS)作用。血脂康能抑制内 皮细胞NF_κ B的激活，具有调脂外的抗AS作用。 　　［关键词］ 胰岛素；血管紧张素II；内皮细胞；核因子-κ B；血脂 康 　　中图分类号：R458＋.5 文献标识码：A 文章编号：1009_816X(2007)03_0 161_03 　　The Effect of Insulin and Angiotensin II on Nuclear Factor-kB Activat ion in human Endothelial cells and the Influence of Xuezhikang Intervention. NIE Zhi-hua, HUANG Shao-lie, HUANG Xiu-zhen, et al. The Second Hospital of Xiamen, Fujian 361026, China 　　［Abstract］ Objective To observe the effect of insulin and angi otensin II (Ang II) on nuclear factor_kB (NF_κ B) activation and the influence of Xuezhikang intervention. Methods Cultured ECV-304 cells (huma n umbilical vein endothelial cell strain) were incubated with 0.5% FBS for 24 h r, then divided randomly into seven groups. Except the control group, the other six groups were treated by insulin (100ul/ml), Ang II (5×10-8mol/ml), ins ulin+Ang II, insulin+Xuezhikang (150ug/ml), Ang II+Xuezhikang, insulin+A ng II+Xuezhikang. Cells were incubated with Xuezhikang for 2 hr before co_incu bated with insulin or /and Ang II in the groups containing Xuezhikang. After co_ incubation for 4 hr, immunocytochemical method was employed to evalu