叠鞘石斛中联苄类化合物的制备工艺及其抗氧化作用研究-药物分析专业论文.docxVIP

万方数据 Abstract Dendrobium nobile is the precious traditional Chinese medicine, which have the effect of nourishing Yin and clearing heat and benefiting stomach fluid. It is widely used in clinic and TCM compounds and mainly contain alkaloids, bibenzyls, phenanthrenes, amino acids, sesquiterpenoids, polysaccharide and other chemical component. Bibenzyl compounds as one of the active ingredient in Dendrobium nobile have broad bioactivities, such as anticancer, antioxidant, antiangiogenic, inhibit NO production. Through review of the literature without report on the research of extraction and purification process. Our research determine to make the bibenzyl compounds from Dendrobinm denneanum as the study object. And to systematic research its’ content determination, extraction process, purification process and antioxidant. So as to lay a foundation for the further development of antioxidant new drug. The main contents and results are as follows: Firstly, to establish the content determination method for bibenzyl compounds and gigantol. Gigantol as the standard substance, using Ultraviolet-visible spectrophotometric determination and HPLC to detect the content of bibenzyl compounds and gigantol. Drawing standard curve for bibenzyl compounds and gigantol, respectively, the regression equation A=0.0153C+0.0054, r=0.9999; Y=813.17X+13.084, r=0.9998, establishing quantitative detection method. Secondly, to research the extraction process of bibenzyl compounds from Dendrobinm denneanum. The extraction rate of bibenzyl compounds, gigantol as the index, to study the single factor of the bibenzyl compounds from Dendrobinm denneanum’s extraction process by solvent reflux. Based on single factor experiment to make the main factors design L9(34) orthogonal test and select out the best process parameters: 70% ethanol, solid-liquid ratio 1g:12ml, 2h each extraction, extraction of 3 times. Again, to extract large amount of bibenzyl compounds, then to research the crude extract purif