脑库建设.pptx

脑库建设与神经病理学竺可青2014-3-11The Art of Brain BankingResearcherDonor脑库用处What can you apply for….. and what do you get1. Brain tissue - different brain areas - paraffin embedded immunostainings - frozen Q-PCR, microarrays, proteomics - fresh cell cultures2. Cerebrospinal fluid - ventricular CSF biomarker reserach, proteomics3. DNA - from blood genetics – coupled to pathology4. Extensive neuropathological report 可以提供临床病理报告 Clinical data are available Information about time of death, post-mortem delay, brain weight, pH CSF5. Special requests possible 研究人员可以提出特殊要求CNS disorders represented in tissue samples collected in BrainNet Europe centresNeurodegenerative disorders and dementia (15 banks)Prion disorders (12 banks)Multiple sclerosis (4 banks)Vascular disorders (6 banks)Epilepsy (3 banks)Psychiatric disorders (5 banks)CNS tumours (6 banks)HIV/AIDS (4 banks)Inflammatory infective disorders (3 banks)Motor neuron disease (1 bank)Parkinson’s disease (2 banks)Huntington’s disease (2 banks)Trauma (1 bank)Metabolic disorders (1 bank)Drug addiction (1 bank)Malformations and congenital disorders (5 banks)Muscle/nerves (2 banks)Control brains (12 banks)BrainNet Europe供者填表对无自主能力者，由家属填表Autopsy protocals 取脑程序controlAlzhemier’s diseaseParkinson’s diseaseFTLD’sMSMSAPSPHDDepressionSchizophrenia样本取材与诊断的标准化神经病理诊断准确的病理诊断至关重要Sometimes senile plaques can already be seen in HE sectionsMisfolded proteins disturbed degradation and aggregationprionsTDP Progranuline mut.Tau Pick’s diseaseTau mutationAbeta in ADMisfolding andaggregationAbeta in CAAnormallyA-synuclein in PDhuntingtinFUSFTLDFUSMut.Rare Sporadic FormsBasophilicInclusionDisease4% ALSAbetaADMCIagingCAA prionsCJD sp.CJD hered.GSSFFIPRPrTDPFTLDspFTLD-TDPPro-granulinmutationsChrom.9 mutationsC orf27TDP-mutationsInclusionsIn 25% ADa-synucleinPDPDDDLBMSATauopathiesFTLD-Tau (chromosome 17T)3 R tau and/or 4 R tauPSP (4 R tau)CBD(4 R tau)Senile tauopa(4 R tau)Argyrophilic grain disease(4 R tau)Pick’s disease(3 R tau)Triplet diseaseProteinsHDhunt