针刺对血管性痴呆大鼠海马突触传递相关信号分子影响.docVIP

针刺对血管性痴呆大鼠海马突触传递相关信号分子影响 　　摘要：目的 观察针刺对血管性痴呆（VD）大鼠海马突触传递相关信号分子蛋白激酶C（PKC）、钙调蛋白激酶（CaMKⅡ）、N-甲基-D-天冬氨酸受体2B亚基（NR2B）的影响，探讨针刺治疗VD的分子机制。方法 采用栓子注入方法制作多发性脑梗死痴呆模型，实验大鼠随机分为正常组、模型组、针刺组和非穴组。针刺组针刺“足三里”治疗，非穴组于肋下髂嵴上10 mm（非穴）处针刺治疗，正常组和模型组大鼠施以与针刺组和非穴组相同强度的捉抓刺激。治疗结束后，ELISA检测PKC活性，Western blot检测CaMKⅡ蛋白表达；免疫组化检测CA1、CA3及DG区NR2B蛋白表达。结果 VD大鼠海马PKC活性、NR2B蛋白表达较正常组显著下降（P0.01）；针刺治疗后，PKC活性明显上升（P0.05），NR2B蛋白表达水平有上升的趋势。各组CaMKⅡ蛋白表达无明显变化。结论 针刺增强海马神经元突触传递信号分子PKC活性，这可能是其治疗VD的重要分子靶点之一。 　　关键词：针刺；血管性痴呆；海马；组织蛋白激酶C；钙蛋白激酶；N-甲基-D-天冬氨酸受体2B亚基；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.07.020 　　中图分类号：R245 文献标识码：A 文章编号：1005-5304（2016）07-0077-05 　　Effects of Acupuncture on Hippocampal Synaptic Transmission Signal Molecules in Rats with Vascular Dementia LI Hui1， WANG Xue-rui2， YANG Jing-wen2， DU Si-qi2， ZHU Wen2， JI Cai-shuo2， LIU Cun-zhi2 （1. Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China； 2. Acupuncture and Moxibustion Department， Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University， Beijing 100010， China） 　　Abstract： Objective To observe the effects of acupuncture on synaptic transmission signal molecules in rats with vascular dementia （VD）， such as PKC， CaMKⅡ and NR2B， and discuss the molecular mechanism of acupuncture treatment for VD. Methods The multi-infarct dementia model was established by injection of emboli into the internal carotid artery. Experimental rats were randomly divided into normal group， model group， acupuncture group and non-acupoint group. For acupuncture group， acupuncture needles were penetrated into bilateral Zusanli. Non-acupoint group was given acupuncture treatment at the bilateral hypochondrium （10 mm above iliac crest）. The rats in normal group and model group were performed to the same amount of capture stimulation as the acupuncture and non-acupoint groups. After treatment， the hippocampal PKC activity was detected by ELISA. Western blot was used to detect CaMKⅡ expression， and the protein expression of NR2B in CA1