和其高密度培养.PDFVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.3 潜在的水体修复菌Bacillus subtilis H001 的筛选 及其高密度培养 刘冬梅，罗彤晖，李昕睿，吴晖，李理，肖性龙，袁琨，赖富饶 （华南理工大学轻工与食品学院，广东广州510640） 摘要：在水产养殖规模日益扩大和集约化程度不断提高时，带来了养殖水体富营养化、亚硝酸盐超标、致病微生物等问题，在 其中引入益生菌，可起到预防和控制的作用。从 10株枯草芽孢杆菌中，筛选出菌株H001 具有较高淀粉酶和蛋白酶酶活，分别为36.7 U/mL 和35.6 U/mL。当麸皮浸汁为40 g/L ，玉米浸汁为15 g/L ，豆粕为20 g/L ，H001 菌落总数（TCN ）对数值分别为12.1，12.1 和 14.0。当玉米浸汁与豆粕粉质量比为2：1 时，麸皮浸汁与豆粕粉的质量比为2:1 时，H001 的TCN 对数值都约为15.0。在模拟水体中， 当亚硝酸盐含量分别为5.0 mg/L、10.0 mg/L、20.0 mg/L、30.0 mg/L、40.0 mg/L 和50.0 mg/L 时，菌株H001 对其降解率分别为95.0% 、 95.0%、84.1%、68.6%、48.5%和44.4%。综上所述，具有较高淀粉酶和蛋白酶酶活的枯草芽孢杆菌H001 ，其培养基经优化后TCN 15 提高到 1.6×10 CFU/mL，优化后培养基组合经济且适合于H001 的高密度培养，H001 是一种潜在的养殖水体修复菌。 关键词：枯草芽孢杆菌；蛋白酶；淀粉酶；高密度培养；养殖水体；亚硝酸盐 文章篇号：1673-9078(2015)3-151-157 DOI: 10.13982/j.mfst.1673-9078.2015.3.026 High-density Culture and Potential Aquatic Remediation Activity of Bacillus subtilis H001 LIU Dong-mei, LUO Tong-hui, LI Xin-rui, WU Hui, LI Li, XIAO Xing-long, YUAN Kun, LAI Fu-rao (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: The increase in scale and degree of intensification of aquaculture has led to several environmental issues, such as eutrophication, excessive nitrite content, and presence of pathogenic microorganisms, in the water bodies used for aquaculture. Introduction of probiotics into such water bodies can help prevent and control these critical issues. Bacillus subtilis strain H001 was selected from among ten screened strains as it showed higher amylase (36.7 U/mL) and protease (35.6 U/mL) activities . The medium components were varied in order to optimize the medium for H001 growth, and the effect was inv