银杏酮酯对离体心肌缺血再灌注损伤血流动力学影响.docVIP

银杏酮酯对离体心肌缺血再灌注损伤血流动力学影响 　　摘要:目的 在离体大鼠心肌缺血再灌注模型上，观察银杏酮酯（GBE50）对大鼠离体心肌缺血再灌注损伤的血流动力学的影响，并探讨其机制。方法 建立改良的Langendorff离体大鼠心脏灌注模型，平衡灌注30 min，缺血30 min，再恢复常速灌注40 min，造成心肌缺血再灌注损伤。用3组不同浓度的银杏酮酯 （12.5 mg/L、25 mg/L、50 mg/L）进行灌流，观察停灌前、再灌5 min、再灌10 min、再灌20 min、再灌40 min时,心率（HR）、左心室收缩峰压平均值（mLVSP）、左心室等容收缩和舒张期压力平均值（±dp/dt【sub】max【/sub】）的变化；冠脉流量（CF）、冠脉流出液超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）的变化。结果 用银杏酮酯灌流缺血的心脏后，与模型组比较,mLVSP、±dp/dt【sub】max【/sub】分别增加；平均冠脉流量增加，冠脉流出液中LDH含量均明显减少（P0.05或P0.01），SOD含量显著升高（P0.05或P0.01）。结论 银杏酮酯对离体的缺血再灌注损伤心脏有保护作用，其作用可能与抗氧化机制有关。 　　关键词:银杏酮酯；缺血再灌注损伤；心功能；大鼠 　　中图分类号：R542.2 R289.5 文献标识码：A 文章编号：1672-1349（2011）06-0698-03Hemodynamic Effects of GBE50 on Ischemia-reperfusion Injury of Isolated Heart in Rats 　　Xie Mixue,Jiang Qi,Sang Yi Miao,et al 　　The Second Clinical Medical College,Zhejiang University of Traditional Chinese Medicine （Hangzhou 310053） 　　Abstract:Objective To investigate the effect of Ginkgo biloba extract 50（GBE50）on ischemia-reperfusion （IR） injury in heart of rats.Methods Langendorff rats model were built,then isolated rat hearts were subjected to reperfusion for 30 min,ischemia for 30 min,and reperfusion for 40 min.GBE50 （12.5 mg/L,25.0 mg/L,50.0 mg/L） was given in reperfusion period.The heart rate（HR）,mean left ventricular systolic pressure （mLVSP）,the maximum change rate of left ventricular pressure rise and fall （±dp/dt）,coronary flow （CF）,superoxide dismutase （SOD）,lactate dehydrogenase （LDH） in coronary sinus effusion were observed.ResultsIn isolated rat heart models,the ±dp/dt【sub】max【/sub】 of the heart was increased.Compared with control group of ischemia-reperfusion,activities of LDH in effluent samples of GBE50 group were lower than that in ischemia-reperfusion group （P0.05 or P0.01）,and activities of SOD in effluent samples of GBE50 group were higher than that in ischemia-reperfusion group （P0.05 or P0.01）.Conclusion GBE50 might have myocardial protective effects during ischemia-reperfusion period. 　　Key words:Ginkgo biloba extract 50ischemia-reperfus