不同无机磷添加水平对混合瘤胃微生物体外发酵的影响.PDF

反刍动物营养研究 不同无机磷添加水平对混合 瘤胃微生物体外发酵的影响 菏泽学院动物科学系/初汉平 李福昌 山东农业大学动物科技学院/王中华 摘 要 采用体外法研究无机磷添加浓度对瘤胃发酵的影响。培养液中设0mg/L、30mg/L、60mg/L、90mg/L、 120mg/L、150mg/L、200mg/L、250mg/L、300mg/L、350mg/L共10个无机磷水平。体外发酵48h后终止培养，测 定培养液的产气量、pH 值、挥发性脂肪酸、干物质降解率、NDF 降解率。结果表明：无机磷添加水平对 48h 总产气 量和 DM 降解率影响显著 (P＜0.05)，随着无机磷添加水平的提高，48h 总产气量和 DM 降解率显著提高，添加水平为 200mg/L 时达到最大值，分别为 44.30mL 和 54.80％，随后下降。瘤胃培养液 pH 值随无机磷添加水平的增加而显著 降低(P＜0.05)，无机磷添加水平为200mg/L 时达到最小值6.35，随后又上升。不同无机磷添加水平对乙酸、丙酸、 丁酸和总VFA产量影响显著(P＜0.05)，无机磷添加水平为200mg/L 时，乙酸、丙酸、丁酸及总VFA产量最高，随后 下降。 关键词 无机磷；瘤胃发酵；产气量；体外法 Abstract The effects of rumen fermentation by adding inorganic P in vitro were studied, concentration of inorganic phosphorus were 0mg/L, 30mg/L, 60mg/L, 90mg/L, 120mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/L and 350mg/L. The gas production, pH, VFA, dry matter and NDF degradation of 48h fermentation in vitro were measured. The results as follow: different inorganic P levels signifi cantly infl uenced the gas production and DM digestibility by 48h fermentation in vitro ＜ (P 0.05). The gas production and DM digestibility increased and reached its maximum when inorganic P was 200mg/L ＜ and then declining. pH was signifi cantly infl uenced by different inorganic phosphorus levels(P 0.05). pH decreased when ＜ inorganic P levels increased(P 0.05)and reached its minimum when inorganic P was 200mg/L. Acetate, propionate, butyrate ＜ acid and total VFA production were signifi cantly effected by the concentration of inorganic phosphorus(P 0.05).Acetate, propionate, butyrate acid and total VFA production were the highest when the concentration of inorganic phosphorus was 200mg/L. ； ； ； Keywor