东乐膏指纹图谱研究和中药及其土壤中有机氯农药残留量的测定-分析化学专业论文.docxVIP

摘 要 摘 要 本文共分三章 第一章 文献综述 概括了中药指纹图谱的定义和属性 总结了中药指纹图 谱研究的意义和作用 详细介绍了这一领域国内外研究现状 探讨了研究中药指 纹图谱的技术问题 引用文献 101 篇 第二章 本研究以东乐膏为载体 初步研究了中药橡胶膏剂指纹图谱的分析 方法 采用 GC HPLC 相结合的方式对其中不同性质的成分进行研究 可以较为全 面地反映东乐膏中的化学组成 利用 CGC 法对中药橡胶膏剂东乐膏中挥发性成分进行了分析 建立了东乐膏 GC 指纹图谱 确定 8 个色谱峰作为特征指纹峰 并对东乐膏中的主要挥发性成分 冰片的含量进行了快速分离测定 分析周期为 6min 线性范围为 40 400mg/L 线性相关系数为 0.9994 利用 HPLC 法对中药橡胶膏剂东乐膏进行了分离 分析 建立 HPLC 指纹图谱 色谱条件 Hypersil ODS2 色谱柱4.6 250 mm 5 m 甲醇-1%醋酸体积百分比浓度为流动相 流速 1.2 mL/min 检测波长 254 nm 该方法简便 准确 重现性好 符合相关技术要求 第三章 应用 CGC 法对白芍 白芷及其所种植的土壤中有机氯农药残留的相 关性进行了初步研究 样品以石油醚+丙酮(3:1)在索氏提取器中提取 提取液以 浓硫酸净化 样品采用 DB-5 弹性石英毛细管柱分离 ECD 检测 该方法简便 稳 定 实用 应用此法考察了土壤中农药残留量对相关种植中草药的影响 关键词 中药指纹图谱 东乐膏 GC HPLC 有机氯农药 ABSTRACT ABSTRACT This paper consists of three chapters. In CHAPTER ONE, literatures and reviews which contains definitions and attributions of fingerprint of Chinese medicine and the importance and effects of researching are summarized. Research progress in China and abroad are introduced in detailed. The technological problems of research of fingerprint of Chinese medicine are discussed. 101 pieces of literatures are cited. In CHAPTER TWO, Donglegao is the carrier of this study. Analytical method of the fingerprint of the traditional Chinese rubber electuary medicine is studied primarily. Integration method of GC and HPLC is employed in the study of different character contents and chemical components in Donglegao can be reflected overall. The fingerprint of Donglegao which is a traditional Chinese rubber electuary medicine has been studied and provides the quality information by capillary gas chromatography. The samples were pretreated by means of ultrasonic wave extraction with alcohol. The capillary column was DB-WAX ( 30m 0.25mm 0.25 m J W Scientific). The content of borneolum in Donglegao was quickly determined. The analytical period was only 6min. The linear ranges were at least within 40-400mg/L ( R= 0.9994, n = 5). On the other hand, the fingerprint of Donglegao has been studied and provide