一株泡叶藻降解菌的筛选及其发酵条件优化Isolationand.PDF

Simpo PDF Merge and Split Unregistered Version - 山东农业大学学报(自然科学版),2014,45(4):515-521 VOL.45NO.42014 JournalofShandongAgriculturalUniversity(NaturalScienceEdition) doi:10.3969/j.issn.1000-2324.2014.04.007 一株泡叶藻降解菌的筛选及其发酵条件优化 * 刘 露公春艳赵宏涛王 鹏李园园, , , , 青岛明月蓝海生物科技有限公司, 山东 青岛 266400 摘 要: 为了利用微生物降解泡叶藻，本研究以泡叶藻为唯一营养物质从土壤中筛选到一株对泡叶藻有明显降解效果 的菌株，编号2-2。根据其形态学特征、生理生化特性和16SrDNA序列分析鉴定该菌株为巨大芽孢杆菌（Bacillus megaterium）。对菌株的发酵条件进行了优化，最优发酵培养基为：泡叶藻30g/L、酵母粉6g/L和葡萄糖4g/L。摇 瓶最佳培养条件为：250mL 三角瓶装液量50mL，起始pH7.5，发酵温度32℃，发酵48h有效活菌数为2.4×109 CFU/mL。并对发酵液酶活进行了测定，其中纤维素酶活为47.7U/mL，蛋白酶活为3.4U/mL，海藻胶裂解酶活为0.721 U/mL 2-2 。研究结果表明巨大芽孢杆菌 同时具有海藻胶裂解酶、蛋白酶和纤维素酶活性，具有良好的应用前景。 关键词: 泡叶藻降解菌; 巨大芽孢杆菌; 海藻胶裂解酶; 发酵条件 中图法分类号:Q936 文献标识码:A 文章编号:1000-2324(2014)04-0515-07 IsolationandFermentationOptimizationofanAscophyllum NodosumDegradingStrain LI Lu,GONGChun-yan,ZHAOHong-tao,WANGPeng,LIYuan-yuan* QingdaoMingyueBlueOceanBio-technologyCo.Ltd.,Qingdao266400,China Abstract: Tostudyascophyllumnodosum degradatedby microorganism, ahigh efficientmicroorganismwas isolated from soil using ascophyllum nodosum as the sole nutrient. Based on the morphological, physiological characteristics and 16S rDNA sequence, the strain was identified as Bacillus megaterium. The optimized fermentation medium was composed of ascophyllumnodosum 30g/L,yeast extract 6g/Landglucose4 g/L.The optimal cultureconditionwas that the strainwas culturedin250mLshakeflakescontaining25mLmediumwithinitialpH7.5at32℃.After48hfermentation,theeffective 9 numberofviablecellswas2.4×10 CFU/mL,theactivityofcellulosewas47.7U/mL,theactivityofproteasewas3.4U/mL, andtheactivityo