广西壮族与汉族人群Toll样受体4基因启动子区遗传多态性研究.docVIP

广西壮族与汉族人群Toll样受体4基因启动子区遗传多态性研究 　　【关键词】 Toll样受体4;基因多态性;广西 　　中图分类号：R394.5 文献标识码：A DOI：10.3969/j.issn.1003-1383.2018.01.002 　　A study of genetic polymorphism of Toll-like receptor 4 at promoter region in Zhuang and Han population in Guangxi 　　LIU Wenjing1，HE Bin，ZHANG Zhuohua，ZHANG Yanting，CHENG Chuyong，HUANG Zhaohe 　　【Abstract】 Objective To investigate the distribution of polymorphisms of r/T and r/A loci of Toll-like receptor 4（TLR4） at promoter region in Zhuang and Han population in Guangxi，and to explore their differences among various regions and groups. 　　Methods Snapshot SNPs genotyping assays and DNA sequencing methods were used to detect genotype and allele of r/T and r/A loci in TLR4 gene promoter region among 123 healthy cases in Zhuang and Han population in Guangxi，and the distribution difference of genotype and allele frequency between Hapmap-HCB，Hapmap-JPT，Hapmap-CEU and Hapmap-YRI was compared and analyzed. 　　Results Three genotypes（CC，CT and TT） were found in r/T of TLR4 gene at promoter region in Zhuang and Han population in Guangxi with the frequency of 13.82%，44.72% and 41.46% respectively，difference of the three genotypes was statistically significant between genders in Guangxi population（Plt;0.05）.However，there was no statistically significant difference in each allele between different genders in Guangxi population（Pgt;0.05）.Difference of genotype and allele frequencies of r/T between Guangxi population and Hapmap-CEU was statistically significant（Plt;0.05）.Three genotypes（AA，AG and GG） were found in r/A with the frequency of 4.07%，32.52% and 63.41%，respectively.There was no statistically significant difference in each genotype and allele between different genders in Guangxi population（Pgt;0.05），but difference of genotype and allele distribution frequencies r/A between Guangxi population and Hapmap-CEU and Hapmap-YRI was statistically significant（Plt;0.001