豇豆与饭豆种间杂种的获得及其鉴定-安徽农业大学学报.PDF

安徽农业大学学报, 2017, 44(3): 519-524 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x008 网络出版时间：2017/5/24 11:18:06 [URL] /kcms/detail/34.1162.S1118.016.html 豇豆与饭豆种间杂种的获得及其鉴定 * 戴希刚，郭 瑞，潘 磊，曾长立，陈禅友 (江汉大学生命科学学院，湖北省豆类（蔬菜）植物工程技术研究中心，武汉 430056) 摘 要：对豇豆和饭豆进行种间杂交试验，30 个正反交组合中有 3 个杂交组合获得种子，它们分别是豇豆 A65 ×饭豆 B24 、豇豆 A27 ×饭豆 D1 、豇豆 A136 ×饭豆 A163 。对获得的种间杂种及其双亲进行表型性状、生理 指标及分子标记鉴定分析结果表明，豇豆 A136 ×饭豆A163 杂交组合所获杂种为假杂种，其他 2 个杂交组合所获 杂种为真杂种。利用豇豆与饭豆种间杂交将饭豆的有利性状基因导入豇豆中，拓宽豇豆的遗传背景，为豇豆种质资 源的创新与新品种选育提供基础。 关键词：豇豆；饭豆；种间杂交；杂种鉴定；ISSR 中图分类号：S643.4 文献标识码：A 文章编号：1672352X (2017)03051906 Acquisition and identification of interspecific hybrids between cowpea and rice bean DAI Xigang, GUO Rui, PAN Lei, ZENG Changli, CHEN Chanyou (Hubei Province Engineering Research Center for Legume Plants School of Life Sciences, Jianghan University, , Wuhan 430056) Abstract: Interspecific hybridization between cowpea and rice bean was conducted. Three crosses, A65 × B24, A27 × D1, A136 × A163, among 30 reciprocal crosses produced seeds. Differences in phenotypic traits, physiological indicators and molecular markers between interspecific hybrids and their parents were studied. The results showed that A136 ×A163 was false hybrids, while the other two were true hybrids. In this study, the fa- vorable traits in rice bean were transferred to cowpea, which broadens the genetic background of cowpea and pro- vides a basis for germplasm innovation and breeding of cowpea. Key words: cowpea; rice beans; interspecific hybrids; hybrid identification; ISSR (Inter-simple sequence re- peat) 豇豆为豆科蝶形花亚科豇豆属豇豆种，学名为 株。这 2 种方法的致命弱点是原始材料的遗传多样 Vigna unguiculata (L.) Walp.，豇豆属是豆科植物中