《酶法提取猴头菇营养液》毕业学术论文.docVIP

PAGE II l 酶法提取猴头菇营养液 摘 要 研究采用纤维素酶和酸性蛋白酶共同酶解提取猴头菇营养液的较优工艺。以营养液中氨基酸总量、可溶性固形物为考查指标，通过单因素和正交试验，确定猴头菇营养液的较优提取工艺。结果表明：料液比为1:25、纤维素酶质量分数1.50%、酸性蛋白酶质量分数1.00%、酶解温度50℃、酶解时间60min、酶解pH3.5为猴头菇营养液提取工艺的较优参数。验证试验表明该方法所得猴头菇营养液氨基酸总量为0.71%，可溶性固形物为3.5%，比水提法分别提高73%和75%。采用酶解方法，可以明显提高猴头菇营养液中氨基酸总量和可溶性固形物的含量。 关键词：猴头菇；营养液；酸性蛋白酶；纤维素酶 Enzymatic extraction of nutritional liquid from Hericium erinaceus ABSTRACT This paper aimed to optimize the enzymatic extraction of nutritional liquid from hericium erinaceus by enzymatic hydrolysis with both acidic protease and cellulose. By using one-factor-at-a-time and orthogonal array design methods, the optimal extraction conditions of nutritional liquid were determined as follows: hericium erinaceus -to-water ratio of 1:25 (m:m), acidic protease dose of 1.50%, cellulose dose of 1.00%, hydrolysis temperature of 50℃ , hydrolysis time of 60min and hydrolysis pH of 3.5. Under the optimum conditions, the extraction efficiency of total amino acids and soluble subjects wre 73%, 75% higher than the yield of water extraction. Key words: Hericium erinaceus；Nutritional liquid；Acidic protease；Cellulase 目 录 摘 要…………………………………………………………………………………Ⅰ ABSTRACT………………………………………………………………………………Ⅱ 1 前言………………………………………………………………………………………1 2 材料与方法……………………………………………………………………………1 2.1 材料………………………………………………………………………………1 2.2 主要仪器和设备…………………………………………………………………2 2.3 指标测定方法……………………………………………………………………2 2.4 工艺流程…………………………………………………………………2 2.5 技术要点………………………………………………………………2 2.5.1 原料选择………………………………………………………2 2.5.2 烘干………………………………………………………………2 2.5.3 粉碎………………………………………………………………2 2.5.4 酶解………………………………………………………………2 2.5.5 灭酶…………………………………………………………………2 2.5.6 离心……………………………………………………………2 2.6 单因素试验………………………………………………………………………2 2.6.1 酸性蛋白酶添加量的确定…………………………………………2 2.6.2 纤维素酶添加量的确定…………………………………………………3 2.6.3 酶解温度的确定……………………………………………………3 2.6.4 酶解时间的确定……………………………………………………3 2.6.5 酶解pH的确定……………………………………………………3 2.6.6 料液比的确定……………………………………………………3 2.7 正交试验…………………………………………………………………………3 3 试验结果与分