活力比较及部分酶学性质-上海海洋大学学报.PDFVIP

第 15 卷第 2 期 上海水产大学学报 Vo 1. 15 , No.2 JOUHNAL OF SHANGHAI FISHERIES UNIVEHSITY Apr.， 2α)6 2∞6 年 4 月 文章编号: 1004 - 7271 (2006)02 - 0211 一 05 几种霉菌产甲壳素脱乙酷酶 活力比较及部分酶学性质 蒋霞云，周培根，李 燕，王晓辉，党培育 (上海水产大学食品学院，上海 2删90) 摘 要:比较了几种霉菌(毛霉、根霉、山莓和青霉)在对数生长末期和稳定期末期的胞内和胞外甲壳素脱乙毗 酶的活力。研究结果表明:(J)各霉菌的胞内和胞外都具有甲壳素脱乙眈酶活力，而且胞外酶的活力普遍大于 It包内酶的活力; (2) 处于对数生长期末期的毛霉菌株产甲壳素脱乙毗酶活力最高，其胞外酶活力达到了 97.2 :t 4.2 U/g干菌丝体。酶学特性研究表明:该酶的最适温度为 50 C，最适 pH 约为 7.2 ，低温保存稳定性较 差，每 24 小时酶活力下降 30% 以上。 关键词:甲壳素脱乙毗酶;甲壳素;壳聚糖;霉菌 中图分类号:TS 201.2 文献标识码:A Comparisons of activities of chitin deacetylase from mold strains and study of its properties JIANG Xia-yun , ZHOU Pei-gen , LI Yan , WANG Xiao-hui , DANG Pei-yu ( College of Food 5c町eαnd Techrwlogy , 51皿nghai Fisheries University , 5hang归 2则90 ， China) Abstract: Activities of intracellular and intercellular chitin deacetylase were compared produced from several mold strains growing at the end of log phase and stable phase respectively. 叽1e experimental results indicated that ( 1) the activities of chitin deacetylase were found inside and outside the cells , and intcrcellular enz严ne was more active than the intracellu!ar one. And (2) the intercellular enzyme growing at the end of log phase had the highest activity , up to 97.2 士 4.2 U/g drγmycelia ， among mo!d strains. The study of the enzyme properties showed that the optimum temperature and pH were 50 c and 7.2 respectively. It was a!so found that the enzyme had a !ess stabi!ity at 4 c and the decrease in the enzyme activity per 24 hours was more than 30% . Key