桑木层孔菌固体培养条件的研究.PDF

Mycosystema 菌 物 学 报 15 May 2011, 30(3): 486-490 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 桑木层孔菌固体培养条件的研究 1 1* 2 曹春蕾 崔宝凯 包海鹰 1 北京林业大学微生物研究所 北京 100083 2 吉林农业大学中药材学院 长春 130118 摘 要：为了研究不同碳源、氮源、初始 pH 及培养温度对桑木层孔菌菌丝生长的影响，采用固体平板培养法研究了桑木层 孔菌的培养条件，结果表明，该菌在淀粉为碳源时生长速度最快但长势较弱，在以葡萄糖和麦芽糖为碳源时生长速度比淀粉 为碳源时稍慢，但长势最好；最适氮源为酵母浸粉；初始 pH 在 5.5 －8.0 范围内变化时菌丝生长差异不大；培养温度在 28 －33℃范围内对菌丝生长有明显的促进作用。 关键词：桑木层孔菌，碳源，氮源，酸碱度，温度 Solid culture conditions of Phellinus mori 1 1* 2 CAO Chun-Lei CUI Bao-Kai BAO Hai-Ying 1Institute of Microbiology, Beijing Forestry University, Beijing 100083, China 2 College of Chinese Traditional Medicine Materials, Jilin Agricultural University, Changchun 130118, China Abstract: Phellinus mori was a recently described species of Phellinus . In order to investigate the influence of different carbon sources, nitrogen sources, initial pH and temperature on mycelial growth of P. mori, its solid culture conditions were studied. The results showed that starch gave the maximum growth rate with less biomass, and glucose and maltose did the opposite. Yeast extract powder was the optimum nitrogen source. The variation of pH value between 5.5 －8.0 did not affect the mycelial growth significantly, and the optimum temperature for mycelial growth was from 28℃ to 33℃. Key words: Phellinus mori, carbonic source, nitrogen source, pH, temperature 桑木层孔菌 Phellinus mori 由Dai et al. （2008 ） 首先在我国东北及华北地区发现，生长于桑树上 基金项目：中央高校基本科研业务费专项资金（No. BLYX200912 ）；国家自然科学基金（No.） * Corresponding author. E-mail: baokaicui@ 收稿日期: 2010-10-29, 接受日期: 2010-11-29 Vol.30 No.3 487 （戴玉成 2010 ），隶属