冬凤兰组织培养和快繁技术研究.docVIP

冬凤兰组织培养和快繁技术研究 　　摘 要 通过探索冬凤兰的组培技术，为冬凤兰的保护和工厂化生产提供技术支撑。以冬凤兰蒴果为原材料，通过不同激素对比，筛选适宜萌芽培养基，并开展了冬凤兰增殖扩繁、生根壮苗、移栽驯化等一系列研究。结果表明：在培养基MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+CM 15%上，萌发时间最早，萌芽率较高；在改良VW培养基上芽分化效果较MS培养基好，芽分化率高，生长势好；在生根培养基VW+IBA 0.5 mg/L+NAA 1.0 mg/L+卡拉胶10 g/L+活性炭0.4 g/L上，生根率达100%；冬凤兰后期需要采用630或650 mL的组培瓶。 　　关键词 冬凤兰 ；VW培养基 ；组织培养 ；快速繁殖 　　中图分类号 S682.31 文献标识码 A Doi：10.12008/j.issn.1009-2196.2017.10.012 　　Tissue Culture and Rapid Propagation of Cymbidium dayanum 　　DONG Xiaona XU Peiling CHEN Pei DU Limin 　　（Forestry Research Institute of Hainan Province， Haikou， Hainan 571100） 　　Abstract Tissue culture was conducted to provide technical support to conservation and mass production of Cymbidium dayanum. Capsules of C. dayanum were used as material and cultured for plant regeneration. Different hormones were tried in the MS mediums to select a suitable germination medium. The germinated capsules were then cultured for proliferation， rooting， formation of strong rooted plantlets and transfer. The results showed that the capsule germinated the earliest with the highest germination rate when cultured in the MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+CM 15% medium， that the bud differentiation was higher in the improved VW medium than in the MS， with higher growth vigor， that the rooting rate was 100% when the plantlets were cultured in the rooting medium VW+IBA 0.5 mg/L+NAA 1.0 mg/L+carrageenan10 g/L+0.4 g/L， and that the rooted plantlets were then transferred into tissue culture bottles with the bottle size of 630/650 mL. 　　Keywords Cymbidium dayanum ； VW medium ； tissue culture ； rapid propagation 　　冬?P兰（Cymbidium dayanum Rchb. f.）为附生兰，生于海拔300～1 600 m的疏林中树上或沿溪谷旁岩石壁上。海南主要产地为陵水、琼中、白沙等地。冬凤兰总状花序具5～9朵花；花葶侧生，下垂或外弯；萼片和花瓣白色至奶油黄色，中央有1条粟褐色纵带（从基部到上部3/4处），或偶见整个瓣片充满淡枣红色，唇瓣栗色，但褶片和中央裂片为白色；冬凤兰花期为8～12月份[1]。冬凤兰花期持久，株型优美，具有极大的观赏价值。罗远华等[2]采用灼烧法对冬凤兰蒴果进行灭菌处理，认为此法不用杀菌剂且操作简便，是冬凤兰种子理想的灭菌方法。林?x君[3]以澳大利亚进口的冬凤兰花梗中部为外植体进行试管繁殖。目前针对冬凤兰的研究相对较少。本研究通过常规升汞消毒的方法处理蒴果，将种子无菌播种于MS培养基，通过不同激素诱导出原球茎或芽后，采用改良VW和MS 2种培养基进行原球茎或芽的分化培养，