外阴上皮内瘤变和HPV感染型别相关性临床研究.docVIP

外阴上皮内瘤变和HPV感染型别相关性临床研究 　　[摘要] 目的 探讨外阴上皮内瘤变（VIN）中人乳头状瘤病毒（HPV）高危型、低危型的感染情况，以及HPV各亚型在VIN病理中的分布情况、各亚型与VIN分级有无相关性。 方法 以人乳头瘤病毒核酸扩增法分型检测各亚型HPV在100例VIN患者及100例外阴正常皮肤组织中的表达情况，并行统计分析。 结果 VIN组织HPV感染率远高于正常组织（χ2=45.54，P 0.05）。VINⅠ中以HPV58感染为主，VINⅡ、VINⅢ中均以HPV16、HPV18感染为主。经Sperman等级相关分析，HPV16/18感染与VIN等级有相关性（r = 0.65，P 0.05）。 结论 HPV感染与VIN发生、发展可能有关系，HPV感染型别在不同级别VIN中有显著差异，HPV16/18可能与VIN的进展有联系，治疗HPV感染可能预防VIN。 　　[关键词] 外阴上皮内瘤变；人乳头状瘤病毒；高危型；低危型；聚合酶链式反应 　　[中图分类号] R737.35 [文献标识码] A [文章编号] 1673-7210（2012）02（a）-0044-03 　　 　　Related clinical research between vaginal intraepithelial neoplasia and HPV infection type 　　WU Ping 　　Gongming Peoples Hospital of Guangming New District, Guangdong Province, Shenzhen 518106, China 　　[Abstract] Objective To investigate the infect conditions of high-risk type and low-risk type human papilloma virus (HPV) in vaginal intraepithelial neoplasia, each subtype of HPV distribution in the VIN pathology, and whether there being relativity between each subtype and VIN grading. Methods Took 100 VIN patients and 100 normal people, detected the expression with papilloma virus nucleic acid amplification method on their vulvar skin tissues, and then made a statistic analysis. Results The HPV infect rate of VIN tissues was much higher than normal tissues (χ2=45.54, P 0.05). It had been proved that there mainly existed HPV58 infection in VINⅠ; there mainly existed HPV16 infection and HPV18 infection in VINⅡ and VINⅢ,. And relativity had been found between HPV16/18 infection and VIN level by having Sperman grading correlation analysis (r = 0.65, P 0.05). Conclusion It maybe have relation between infection of HPV and occurrence and development of VIN, HPV infect subtype has statistic difference in different grade of VIN, HPV16/18 may have relation with development of VIN, treatment of HPV infection can prevent VIN. 　　[Key words] Vaginal intraepithelial neoplasia; HPV; High-fisk type; Polymerase chain reaction 　　 　　外阴上皮内瘤变（VIN）的发病原因目前还不完全清楚，可能与人乳头状瘤病毒（HPV）感染有关[1