水稻粒形性状的上位性和QE互作效应分析AnalysisofEpistatic-Core.PDF

分子植物育种，2009 年，第7 卷，第4 期，第690-698 页 Molecular Plant Breeding, 2009, Vol.7, No.4, 690-698 研究报告 Research Report 水稻粒形性状的上位性和QE 互作效应分析 * * ** 江良荣 王伟 黄建勋 黄荣裕 郑景生 黄育民 王侯聪 厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室, 厦门, 361005 * 同等贡献作者 ** 通讯作者, hym@ 摘 要 本研究利用基于明恢86×佳辐占水稻重组自交系(recombinant inbred line, RIL)构建的SSR 遗传图 谱，总标记数为131。联合两季的稻米粒长(GL)、粒宽(GW)、长宽比(L/W)表型数据，应用混合线性模型方法进 行QTL 定位，并作加性效应、上位效应以及加性QTL、上位性QTL 与环境(QTL-by-environment, QE) 的互作 效应分析。检测到粒长、粒宽和长宽比的加性效应QTLs 分别为6 个、4 个和4 个，贡献率分别为23.67% 、 21.41%和25.78% ；检测到8 对粒长的上位性QTLs，5 对粒宽的上位性QTLs，2 对长宽比的上位性QTLs，贡 献率分别为 16.75%、22.36%和7.55% ；环境互作检测中，发现共有9 个加性QTLs 和7 对上位性QTLs 与环 境发生了互作。结果表明，上位效应在粒形性状的遗传与加性效应一样起了重要作用，环境互作效应对粒形 性状有一定的影响。 关键词 水稻, 数量性状基因座位, 上位性效应, QE 互作效应 Analysis of Epistatic and QE Interaction Effects of QTLs for Grain Shape in Rice Jiang Liangrong * Wang Wei * Huang Jianxun Huang Rongyu Zheng Jingsheng Huang Yumin ** Wang Houcong Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, 361005 * The authors who contribute equally ** Corresponding author, hym@ DOI: 10.3969/mpb.007.000690 Abstract A linkage map consisting of 131 markers was constructed by using a recombinant inbred line (RIL) population derived from the - rice cross Minghui86 Jiafuzhan. Quantitative trait loci (QTLs) was lo- indica indica × cated with a mixed linear model (QTLmapper1.6) based on the grain shape parameters such as grain length (GL), grain width (GW) and length-width ratio (L/W), and made analysis of additive effects, epistatic, and the interaction effects of QTL-by-environment (QE). Six, four and four QTLs were detected to have significant additive effects for GL, GW and L/W, and the contribution rate were 23.67% 、21.41% and 25.78%, respectively. Eight, five and two pairs of epistatic QTLs with significant additive-by-additive (AA) in