冷冻方式对海湾扇贝闭壳肌大分子物质及组织结构的影响.docx

冷冻方式对海湾扇贝闭壳肌大分子 物质及组织结构的影响 崔瑞颖，焦学芹，祖铁红，张志胜*，崔 波，张欣彩 (河北农业大学食品科技学院,保定 071000) 摘要:以海湾扇贝闭壳肌TBA值s肌原纤维蛋白Ca2+-ATPase活性及组织的显微镜观察为指标, 研究了不同冷冻方式[(-25±1) ?冰箱冷冻s(-35±1) ?冰柜冷冻s(-80±1) ?超低温冷冻和液 氮冷冻]对海湾扇贝闭壳肌大分子物质及组织结构的影响o研究结果表明:相同冷冻方式条件 下,冻藏时间对其TBA值及肌原纤维蛋白Ca2+-ATPase活性影响显著(p0.05):相同冻藏时间条 件下,不同的冷冻方式对海湾扇贝闭壳肌的TBA值影响差异均显著(p0.05),液氮处理对肌原纤 维蛋白Ca2+-ATPase活性影响与其他处理间差异显著(p0.05),其他3种处理间差异不显著o由光 学显微镜下图片可知,液氮处理对海湾扇贝闭壳肌肌肉纤维的影响最小,与新鲜闭壳肌的结构 最相近,(-25±1) ?冰箱冷冻对闭壳肌肌肉纤维结构破坏最大o因此,选择(-35±1) ?冰柜冷冻 海湾扇贝闭壳肌对大分子物质及组织结构的变化影响较好o 关键词:冷冻方式:海湾扇贝:闭壳肌:大分子:结构 中图分类号:TS 254.4 文献标志码:A 文章编号:1005-9989(2014)02-0134-05 Effects of different freezing processes on biomacromolecules and structure of Argopecten irradiams muscle CUI Rui-ying, JIAO Xue-qin, ZU Tie-hong, ZHANG Zhi-sheng*, CUI Bo, ZHANG Xin-cai (Food Science and Technology College, Agricultural University of Hebei, Baoding 071000) Abstract: Taking TBA, the Ca2+-ATPase activity in myofibrillar protein and the structure of Argopecten irradiams muscle as the indexes, the effects of 4 different freezing processes, such as (-25±1) ℃ refrigerator freezing, (-35±1) ℃ refrigerator freezing, (-80±1) ℃ ultra-low temperature freezing and liquid nitrogen freezing, on biomacromolecules and structure of Argopecten irradiams muscle were studied. Results showed that: At the same freezing process, TBA and the Ca2+-ATPase activity in myofibrillar protein change signifienatly at different frozen storage time (p＜0.05); at the same frozen storage time, TBA change signifienatly at different freezing processes (p＜0.05), the Ca2+-ATPase activity in myofibrillar protein change signifienatly between liquid nitrogen freezing and other freezing processes (p＜0.05). By optical microscope images, Argopecten irradiams muscle structure by liquid nitrogen freezing similar to the fresh muscle structure. The change of muscle fiber structure is the largest by (-25±1) ℃ refrigerator freezing of Argopecten irradiams muscle. So, (-35±1) ℃ refrigerator freezing was be