（毕业学术论文设计）-木蹄层孔菌Lac的酶学性质研究论文.docVIP

PAGE l 木蹄层孔菌Lac的酶学性质研究 摘 要 本文对木蹄层孔菌漆酶的酶学性质进行了研究。结果如下：以ABTS作为底物，酶反应的最适温度为50℃；最适pH值为3；在40°C以下保持酶活力相对稳定，50°C酶活性基本全部丧失；在pH值3.5~4范围内保持相对较高的酶活力。10mM/L的Ba2+、Ca2+、Co+、Fe3+、Na+、K+这六个金属离子对漆酶酶活有较强的抑制作用，其中Fe3+的抑制作用最强，而Mg2+，Cu2+对两种漆酶均有激活作用。以ABTS为底物时，漆酶的Km值为0.05mmol/L。 关键词：木蹄层孔菌，漆酶，酶学性质  ENZYMATIC PROPERTIES STUDY OF tinder fungus LACCASE ABSTRACT In this paper, the tinder fungus enzymology properties of laccase were studied. Results are as follows: to ABTS as substrate, the enzyme reaction of the optimum temperature is 50 ℃; The optimal pH value is 3; Enzyme activity stabilization are under 40 ° C, basic all lose enzyme activity under 50 ° C; Within the scope of the pH value of 3.5 ~ 4 remained relatively high enzyme activity. 10 mm/L Co+, Ca2+, Ba2+, Fe3+, Na+, K+ the six metal ions on laccase enzyme activity had a stronger inhibitory effect, one of the strongest inhibitory effect of Fe3+, and Mg 2+, Cu2+ effect on two kinds of laccase are activated. ABTS as substrates, laccase Km value tendency for 0.05 L. Key words: tinder fungus，laccase，enzymatic properties  目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc355812761 HYPERLINK \l _Toc355812762 第1章 绪论 PAGEREF _Toc355812762 \h 1 HYPERLINK \l _Toc355812763 1.1木蹄层孔菌国内外研究进展 PAGEREF _Toc355812763 \h 1 HYPERLINK \l _Toc355812764 1.2漆酶国内外研究进展 PAGEREF _Toc355812764 \h 1 HYPERLINK \l _Toc355812765 1.2.1漆酶理化性质 PAGEREF _Toc355812765 \h 2 HYPERLINK \l _Toc355812766 1.2.2漆酶的应用 PAGEREF _Toc355812766 \h 3 HYPERLINK \l _Toc355812767 第2 章 材料和方法 PAGEREF _Toc355812767 \h 4 HYPERLINK \l _Toc355812768 2.1实验材料与仪器 PAGEREF _Toc355812768 \h 4 HYPERLINK \l _Toc355812769 2.1.1菌种 PAGEREF _Toc355812769 \h 4 HYPERLINK \l _Toc355812770 2.1.2实验材料、试剂 PAGEREF _Toc355812770 \h 4 HYPERLINK \l _Toc355812771 2.1.3实验仪器 PAGEREF _Toc355812771 \h 5 HYPERLINK \l _Toc355812772 2.2实验方法 PAGEREF _Toc355812772 \h 5 HYPERLINK \l _Toc355812773 2.2.1木蹄层孔菌的培养 PAGEREF _Toc355812773 \h 5 HYPERLINK \l _Toc35581277