年龄对SAMP8小鼠不同脑区NSF含量影响.PDFVIP

安徽农业大学学报, 2011, 38(5): 680-682 Journal of Anhui Agricultural University [DOI]CNKI：34-1162/S1432.022 网络出版时间：2011-08-29 14:32:20 [URL]http:// /kcms/detail/34.1162.S1432.022.html 年龄对 SAMP8 小鼠不同脑区 NSF 含量的影响 * 马 汉，陈贵海 ，黄 玲，李学伟，王 飞，杨启纲 (安徽医科大学第一附属医院神经内科，合肥 230032) 摘 要：采用免疫印迹技术检测NSF 蛋白和内参蛋白GAPDH 在SAMP8 小鼠背侧海马、腹侧海马、额叶、顶 叶和嗅球的表达。结果显示在背海马、额叶和顶叶中，13 月龄SAMP8 小鼠NSF 的相对含量显著高于5 月龄（Ps 0.05 ），且13 月龄背侧海马NSF 含量也高于9 月龄鼠（P0.05 ），而在腹海马和嗅球NSF 的含量在各个年龄段间差 异无统计学意义（Ps 0.05 ）。以上结果提示在正常衰老过程中，SAMP8 小鼠NSF 含量在脑内某些区域呈现年龄相 关性增高。 关键词：衰老；NSF ；小鼠；免疫印迹法 中图分类号：R339. 38 ；R338. 13 文献标识码：A 文章编号：1672−352X (2011)05−0680−03 Influence of age on the levels of NSF in different brain regions in SAMP8 mice MA Han, CHEN Gui-hai, HUANG Ling, LI Xue-wei,WANG Fei, YANG Qi-gang (Department of Neurology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032) Abstract: The NSF(N-ethylmaleimide-sensitive fusion protein) and GAPDH (reference protein) in dorsal hippocampus, ventral hippocampus, frontal lobe, parietal lobe and olfactory bulb were detected by western blot. The relative amount of the NSF in the dorsal hippocampus, frontal lobe and parietal lobe in the 13-month-old mice was significantly higher than that in the young mice (Ps 0.05), and the amount of NSF in the 13-month-old mice in the dorsal hippocampus was higher than that in the 9-month-old mice (P 0.05); the relative content of NSF in the SAMP8 mice in the ventral hippocampus and olfactory bulb had no significant age-related changes(Ps 0.05). Our results suggested that during the lifetime, the amount of NSF increases with aging in some brain re- gions of SAMP8 mice. Key words: aging; NSF; mouse; western blot 衰老是生物体随着时间推移而自发的维持细胞