六味葛蓝降脂片大鼠30d喂养试验的研究.docVIP

六味葛蓝降脂片大鼠30d喂养试验的研究 　　[摘要] 目的 通过大鼠30 d喂养试验，探讨六味葛蓝降脂片的安全性。 方法 选取80只大鼠，随机分为3个剂量组和1个对照组（共4组），每组20只，雌、雄各10只。剂量组给予15.0、10.0、5.0 g/（kg?BW）的剂量（人体可能摄入量的150、100、50倍），对照组灌服等体积蒸馏水，每天灌胃1次，连续30 d后对大鼠各项指标进行观察。 结果 各剂量组与对照组在试验过程中均生长良好，无死亡，未发现异常体征。各剂量组的体重增长率、食物利用率、血液学指标、血液生化指标、脏/体比值等指标与对照组比较，差异无统计学意义（P0.05）。 结论 在本实验的剂量范围内，六味葛蓝降脂片大鼠30 d喂养未见短期的毒副作用。 　　[关键词] 六味葛蓝降脂片；毒理学安全性；实验研究 　　[中图分类号] R-332 [文献标识码] A [文章编号] 1674-4721（2016）03（c）-0015-04 　　[Abstract] Objective To explore the safety of Liuwei Gelan Lipid-decreasing tablet by 30-day feeding in rats. Methods 80 rats were selected and randomly divided into 3 groups with dosages and the control group.Male and female rats were equally divided.In these dosage groups，15.0，10.0 and 5.0 g/（kg?BW） were provided，which were 150，100 and 50 times of dosages taken by human body.In the control group，the same volume of distilled water was supplied.Gavage was performed once per day.The each index from rats was observed and tested after 30 continuing days of experiment. Results Rats were all well grown without death in the process of experiment in the control group and each dosage group.No abnormal sign was detected.There was no significant difference in the weight growth rate，food utilization rate，haematological inde，hematologic and biochemical indices，viscera/body ratio between these among 3 dosage groups and the control group （P0.05）. Conclusion Within dosages tested in the experiment，no short-term toxic and side effect is detected after 30-day feeding in rats by Liuwei Gelan Lipid-decreasing tablets. 　　[Key words] Liuwei Gelan Lipid-decreasing tablet；Toxicological safety；30-day feeding 　　高脂血症（hyperlipaemia，HLP）是一种常见病、多发病，常诱发冠心病、动脉硬化、脂肪肝、糖尿病、肥胖等症，严重危害人类的身体健康。随着人们生活水平的不断提高和社会人口老龄化，高脂血症的发病率呈明显上升和年轻化趋势。目前，用于治疗高脂血症的西药（如他汀类、贝特类、阿而莫司类、烟酸类、胆酸螯合剂等）因起效快且疗效肯定而受到大家的青睐，但是西药存在靶点单一、降脂作用单一及不同程度的毒副作用等局限性。中医在治疗高脂血症、防止心脑血管疾病发生等方面有着多药理活性、多靶点、多环节协调综合降脂的独特优势，其疗效肯定、持续，副作用小[1-11]。六味葛蓝降脂片为广西中医药大学制药厂拟开发的具有辅助降血脂功效的保健食品，处方遵循中医药君臣佐使的配伍原则，由绞股蓝、西洋参、枸杞子、决明子等药食同源药材配伍而成，具有益气健脾