不同温度驯化策略诱导优雅蝈螽海藻糖合成酶基因表达-应用昆虫学报.PDF

Chinese Journal of Applied Entomology 2017, 54(1): 5667. DOI: 10.7679/j.issn.20951353.2017.006 不同温度驯化策略诱导优雅蝈螽海藻糖 合成酶基因表达及血淋巴糖类含量变化* ** *** 骞蕾阳 寇晓艳 董泽华 常岩林 周志军 071002 摘 要 【目的】 Gampsocleis gratiosa Trehalose- 6-phosphate synthase, TPS 【方法】 TPS cDNA PCR TPS 25 ℃ 【结果】 TPS GgTPSGenBank KU578006 3 225 bp2 430 bp 5′-5′-untranslated region, 5′-UTR153 bp 3′- 3′-untranslated region, 3′-UTR642 bp809 Mw91.35 ku pI6.28TPS 15℃ 25 ℃ 0 ℃25 ℃0 ℃ 10℃25 ℃ TPS 40 ℃25 ℃ TPS 15℃20 ℃25 ℃ 0 ℃25 ℃0 ℃10℃20 ℃25 ℃ TPS 30 ℃ 45 ℃ 25 ℃ 40 ℃ 25 ℃40 ℃45 ℃25 ℃TPS 15℃40 ℃ 40 ℃ 45 ℃ 10℃ 20 ℃ 30 ℃ 【结论】 TPS TPS 关键词 TPS PCR Effects of different temperature regimes on the expression of the trehalose-6-phosphate synthase gene and haemolymph sugar content in Gampsocleis gratiosa ** *** QIAN Lei-Yang KOU Xiao-Yan DONG Ze-Hua CHANG Yan-Lin ZHOU Zhi-Jun (Key Laboratory of Invertebrate Systematics and Application of Hebei Province, College of Life Science, Hebei University, Baoding 071002, China) Abstract [Objectives] To ascertain the effects of rapid vs gradual change in temperature on trehalose-6-phosphate synthase (TPS) gene expression levels, and trehalose, and total sugar, content, in the haemolymph of Gampsocleis gratiosa. [Methods] Based on the G. gratiosa transcriptome sequencing data set, the full cDNA of the G. gratiosa TPS gene was *Supported project **First authorE-mail qianleiyanghb@163.com ***Corresponding authorE-mail zhijunzhou@163.com