金丝桃花药发育的细胞化学研究-中国细胞生物学学报.PDF

中国细胞生物学学报 Chinese Journal of Cell Biology 2016, 38(6): 669–675 DOI: 10.11844/cj cb.2016.06.0060 金丝桃花药发育的细胞化学研究 魏冬梅1,2* 杨淑娟3 杨日升2 1 2 3 ( 台州学院生命科学学院, 台州318000; 浙江康能食品有限公司, 天台317200; 宁夏大学生命科学学院, 银川750021) 摘要 花药发育过程中多糖和脂滴物质的代谢具有时空变化特征。不同植物的代谢特征有 很大差异。该研究采用组织化学的方法对金丝桃花药发育中多糖和脂滴的动态分布进行观察。结 果发现, 多糖和脂滴的分布具有一定的规律。在造孢细胞时期, 维管束周围的药隔薄壁细胞和花药 壁的表皮、药室内壁和中层细胞中积累淀粉多糖, 而绒毡层细胞中没有淀粉多糖。在小孢子早期, 绒毡层细胞中出现淀粉多糖并且数量逐渐增加; 此时, 单核中位期小孢子中也出现淀粉多糖。到小 孢子晚期, 绒毡层细胞中积累的淀粉多糖消失, 同时出现脂滴; 晚期小孢子中也开始出现脂滴。到 二胞花粉时期, 绒毡层细胞原位解体, 花粉中脂滴数量增加。金丝桃相当数量的花粉在小孢子时期 发生异常。开花时, 大多数花粉内为空腔, 没有淀粉多糖和脂滴积累, 花粉败育。发育正常的花粉 内充满了淀粉多糖和脂滴。 关键词 金丝桃; 花药发育; 细胞化学; 淀粉多糖; 脂滴 Cytochemical Study on the Anther Development of Hypericum monogynum L. Wei Dongmei1,2*, Yang Shujuan3, Yang Risheng2 1 2 ( School of Life Science, Taizhou University, Taizhou 318000, China; Zhejiang Kangneng Foodstuffs Co., Ltd., 3 Tiantai 317200, China; School of Life Science, Ningxia University, Yinchuan 750021, China) Abstract The metabolisms of polysaccharides and lipids have temporal and spatial characteristic during the anther development. The metabolic characteristics of polysaccharides and lipids differ with plant species. The dynamic distribution of polysaccharides and lipids was observed in the anther developmental processes of Hyperi- cum monogynum L. by histochemical methods. The results showed the distribution of polysaccharides and lipids displayed some characters. At the stage of sporogenous cell, a lot of starch polysaccharides were accumulated in parenchyma cells around vascular bu