多基因联合提高结直肠癌甲基化检测阳性率的研究外科学(普外科)专业论文.docxVIP

安徽 安徽医科大学硕士学位论文 PAGE PAGE 1 英文缩略词表 英文缩写 英文名称 中文名称 CRC Colorectal Cancer 结直肠癌 DNA Deoxyribose Nucleic Acid 脱氧核糖核酸 HPP1 Hyperplastic Polyposis Protein gene 1 增生性息肉蛋白 MSP MGMT Methylation-specific PCR Methylguanine-DNA Methyltransferase 甲基化特异性聚合酶链反应 甲基鸟嘌呤甲基转移 PCR Polymerase Chain Reaction 聚合酶链式反应 SFRP1 TGF Secreted frizzled related protein 1 Transforming Growth Factor 分泌型卷曲相关蛋白1 转化生长因子 多基因联合提高结直肠癌甲基化检测阳性率的研究 【摘要】 目的 中文摘要 研究联合检测Vimentin、sFRP1和HPP1基因甲基化对提高结直肠癌甲基化阳性 率的意义。 方法 收集90例结直肠癌、60例腺瘤性息肉患者及20例结直肠正常组织标本，提取 组织标本的DNA，采用甲基化特异性PCR（MSP）检测Vimentin、sFRP1和HPP1基因 甲基化状态，分析其与结直肠癌的关系及甲基化阳性率。 结果 结 直 肠 癌 患 者 组 织 DNA 中 Vimentin 、 sFRP1 和 HPP1 基 因 甲 基 化 率 分 别 为 66.7%、68.9%、72.2%。腺瘤患者分别为53.3%、55.0%、50.0%。正常对照组分别 为0、0、5.0%。结直肠癌患者的3个基因甲基化阳性率均高于腺瘤患者和正常对照 组（P0.05）。3个基因甲基化联合检测诊断结直肠癌、腺瘤的阳性率分别为93.3%、 76.7%，高于单个基因的甲基化阳性率（P0.05）。3个基因甲基化状态与结直肠 癌患者的性别、年龄、肿瘤部位、淋巴结转移、远处转移及TNM分期无关（P0.05）。 结论 Vimentin、sFRP1和HPP1基因启动子甲基化水平在结直肠癌患者中升高，其联 合检测明显提高了结直肠癌甲基化检测阳性率，有可能成为结直肠癌早期诊断的 甲基化检测方案。 关键词 结直肠癌 甲基化 Multi-gene methylation testing increased the positive methylation rate in colorectal cancer Abstract 【Abstract】 Objective To study the value of detection the methylation of Vimentin、sFRP1 and HPP1 gene in colorectal cancer. Methods 90 patients with colorectal cancer, 60 patients with adenomatous polyp and 20 normal people, were included in the study. DNA was extracted from tissue samples, and the methylation status of Vimentin, sFRP1 and HPP1 gene were detected by Methylation-specific PCR (MSP). The relation between clinicopathologic features of colorectal cancer and gene methylation were analyzed. Results The methylation frequencies of Vimentin, sFRP1 and HPP1 were 66.7%、 68.9%、72.2% in colorectal cancer, 53.3%、55.0%、50.0% in colorectal adenomas, and 0、0、5.0% in healthy controls, respectively. The methylation of each of the three genes in colorectal cancer were higher than colorectal adenomas and healthy controls (P0.05). The diagnostic sensitivity by combining three methylation marks was 93.3% in colore