二甲双胍上调血清可溶性瘦素受体水平及其机制的初步探讨-内科学(内分泌与代谢病)专业论文.docxVIP

缩略语表 英文缩写 BMI 英文全称 body mass index 中文全称 体重指数 BSA bovine serum albumin 牛血清白蛋白 cDNA complementary DNA 互补 DNA CD control diet 普通对照饮食 cm centimeter 厘米 ddH2O double distilled water 双蒸水 d day 天 DEPC diethylpyrocarbonate 焦碳酸二乙酯 DM diabetes mellitus 糖尿病 DMEM dulbecco′s modified eagle medium DMEM 培养基 DMSO dimethyl sulfoxide 二甲基亚砜 DNA deoxyribonucleic acid 脱氧核糖核酸 FBS fetal bovine serum 胎牛血清 Fins fasting serum insulin 空腹血浆胰岛素 FPG fasting plasma glucose 空腹血糖 GTT glucose tolerance test 葡萄糖耐量测试 GA glycated albumin 糖化白蛋白 g gram 克 h hour 小时 HbAlc glycosylated hemoglobin 糖化血红蛋白 HFD high-fat diet 高脂饮食 IGT impaired glucose tolerance 糖耐量受损 INS Insulin 胰岛素 IR insulin resistance 胰岛素抵抗 ITT insulin tolerance test 胰岛素释放实验 kDa kilo-dalton 千道尔顿 kg kilogram 千克 1 mRNA mg min ml mM ng nm OD PBS PCR pH rpm s sOB-R SPSS T2DM μl μg wk messenger ribonucleic acid millgram minute millilitre mmol/L nanogram nanometer optical density value phosphate buffered solution polymerase chain reaction hydrogen ion concentration rotations per minute second soluble leptin receptor statistical package for social science type 2 diabetes mellitus microlitre microgram week 信使核糖核酸 毫克 分钟 毫升 毫摩尔每升 纳克 纳米 吸光度值 磷酸盐缓冲液 聚合酶链反应 酸碱度 转/分 秒 可溶性瘦素受体 社会科学统计软件包 2 型糖尿病 微升 微克 周 2 Metformin upregulates serum soluble leptin receptor and its underlying mechanism Abstract Background and Objective: Soluble leptin receptor (sOB-R) is the product of proteolysis of membrane leptin receptors at its extracellular region. As the major leptin binding protein in circulation, sOB-R is important in regulation of the free/bound leptin index, thus plays a crucial role in regulating the bioavailability and physiological function of leptin. Accumulating epidemiological studies show that level of sOB-R in plasma is negatively correlated to disease states associated with abnormal leptin levels, such as Obese, T2DM and colorectal cancer. In a prospective investigation into the association of sOB-R with risk of metabolic disorders in a healthy population