凡纳滨对虾(Litopenaeus vannamei)和罗氏沼虾(Macrobrachium rosenbergii)卵黄蛋白原mRNA合成部位的初步分析-水产养殖专业论文.docxVIP

巢在整个发育过程中对卵黄蛋白原合成的贡献比较小，分别为 1.0, 1.3, 1.7, 4.8, 1.5, 除成熟期外，其他各期差异不显著。同时，Northern 杂交结果表明在次级卵 黄发生期和成熟期的肝胰腺中有较明显的杂交信号的出现，并且杂交信号随着卵 巢的发育而增强。在卵巢中则没有检测到非常明显的杂交信号的出现。这与荧光 定量 PCR 中的结果吻合。与凡纳滨对虾相似，罗氏沼虾的卵黄蛋白原的相对表达 量也是随着卵巢的发育不断增加，在成熟期达到最大，抱卵期急剧减少。罗氏沼 虾的卵巢合成卵黄蛋白原的能力与肝胰腺相比很小，这也是罗氏沼虾和凡纳滨对 虾在卵黄蛋白原合成中的主要不同点。 在罗氏沼虾中大部分卵黄蛋白原（87.7％）是由肝胰腺合成的，然而在凡纳 滨对虾的卵黄蛋白原合成中卵巢和肝胰腺的合成能力几乎相同。 关键词：凡纳滨对虾，罗氏沼虾，卵黄蛋白原，合成部位，mRNA 表达，卵巢， 肝胰腺 Study on site of vitellogenin synthesis in the shrimp Litopenaeus vannamei and Macrobrachium rosenbergii Abstract With the development of aquaculture techniques and the large scale of aquaculture of shrimps and crabs, it is critical to obtain enough eggs with high quality. In eucyphidea shrimps such as Macrobrachium rosenbergii, the ovaries are ready to be mature. They could continuously lay eggs for several times. Artificial breeding factories are also common. For Litopenaeus vannamei, a representative shrimp of the penaeidae, eyestalk ablation is often necessary to induce the ovary to develop. The reason for this difference between the two groups of shrimp has not been understood for a long time. During ovarian maturation, yolk protein accumulation is the most important event. The differences of yolk protein synthesis between the two groups of shrimp may be related to the different mechanisms of oocyte maturation. At present study, the accurate site for vitellogenin (the precursor of vitellin which is the major component of yolk protein) synthesis was first investigated in two representative shrimp species Litopenaeus vannamei and Macrobrachium rosenbergii. Based on the references and our histological study, the ovarian development was divided into six stages in L. vannamei (oogonium proliferation, previtellogenic, primary vitellogenic, secondary vitellogenic, mature, and recovery stages) and five stages in M. rosenbergii (previtellogenic, primary vitellogenic, secondary vitellogenic, mature, and spent stages). Real time PCR method was used to compare the expression of v