二十味排毒汤对痤疮丙酸杆菌及金黄地鼠皮脂腺斑影响的研究-中医外科学专业论文.docxVIP

中文摘要 目的：本研究拟通过动物实验阐明二十味排毒汤的抗皮脂腺增生作用，并 了解其对痤疮丙酸杆菌的体外抑制作用，为临床应用提供实验依据。 方法：1、采用随机对照的研究方案分别检测二十味排毒汤药液和丹参酮胶 囊药液的最低抑菌浓度（MIC）。2、将 50 只雄性金黄地鼠以随机分为模型组、 二十味排毒汤大、中、小剂量组和丹参酮胶囊阳性对照组，分别给予灌胃处理。 4 周后测量右侧皮脂腺斑面积。并观察皮脂腺斑的显微结构。 结果：在二十味排毒汤的体外抑菌实验中，与模型对照组比较，其最低抑 菌浓度（MIC）为 31.25 mg/ml，丹参酮最低抑菌浓度（MIC）为 62.5 mg/ml。 在抑制皮脂腺的大小方面：二十味排毒汤大、中、小剂量组、丹参酮对照组与 模型组比较均有明显差异（p＜0.05），而大剂量组与丹参酮对照组比较表现出 明显差异（p＜0.05），说明在同等条件下，二十味排毒汤中、小剂量组在控制 金黄地鼠皮脂腺斑大小的作用与丹参酮相当，而大剂量组比丹参酮的作用明显 增强。 在抑制金黄地鼠皮脂腺厚度方面：从二十味排毒汤小剂量组开始，与模型 组和对照组比较均表现明显抑制作用（p＜0.05），此抑制作用与剂量浓度成正 比。 结论：1、二十味排毒汤对痤疮丙酸杆菌有较强的体外抑菌作用，最低抑菌 浓度（MIC）为 31.25 mg/ml，作用优于丹参酮胶囊；2、二十味排毒汤具有良好 的抑制皮脂腺增生的作用。此作用与二十味排毒汤的使用剂量成正比关系，大 剂量的作用则明显优于丹参酮胶囊； 关键词：二十味排毒汤；痤疮； 痤疮丙酸杆菌； 动物模型； 皮脂腺斑 Abstract Objective Purpose: This study was verified by animal experiments in which we used twenty-flavor- detox-soup(TFDS) to test its role of anti-sebaceous-hyperplasia, and learned its effects on Propionibacterium acnes in vitro, so we could provide experimental basis for clinical application. Method: 1, Using randomized study plan respectively TFDS liquor taste detoxification soup and the lowest percentage capsule inhibitory concentration (MIC). 2，The male hamsters were divided into model group, TFDS of high-dose group (Ⅰgroup), middle-dose group (Ⅱgroup), low-dose group（Ⅲgroup）, and tanshinone capsule positive control group. Different concentrations of TFDS and tanshinone liquid capsules were administered to the animals. After 4 weeks the maximum diameter measured on the right patch (DT) and the maximum longitudinal diameter (DL). Taken hamster flank sebaceous spots organizations, sebaceous glands were observed in the light spot of the microstructure. Results: The minimum inhibitory concentration (MIC) of TFDS was 31.25 mg/ml, while tanshinone’s minimum inhibitory concentration (MIC) was 62.5 mg/ml. In inhibiting the size of sebaceous glands, the medium-dose and the small-dose group and model group and control group of tanshinone had none significant differences (p＞0.05), but the high-do