柚子皮挥发油提取及成分测定中医中药.docVIP

本 科 毕 业 设 计 (论 文) 柚子皮挥发油提取及成分测定 The extraction and content determination of volatile oil from Pomelo peel 学 院： 化学工程学院 专业班级： 化学工程与工艺 DZ化工101 学生姓名： 王文静 学 号： 2012140043 指导教师： 夏海涛（教授） 2014年6月 毕业设计（论文）中文摘要 柚子皮的挥发油提取及成分测定 摘 要：柚子皮挥发油的提取实验使用水蒸气蒸馏法，采用常温浸提技术，以蒸馏水为浸提试剂，对影响挥发油提取量的因素进行研究。实验表明挥发油的提取量主要取决于浸提时间、料水比、提取时间等影响因素。通过一系列实验表明， 在8小时的浸泡时间，1:26g的料水比以及12小时的提取时间的条件下，提取的挥发油最多，是最适宜的实验提取条件。利用气相色谱质谱联用技术对柚子皮化学成分进行分离鉴定,测定挥发油成分，分析挥发油化学性质。共检测出62种不同组分，其中31种组分占挥发油含量的百分之五十。其主要成分是β-蒎烯、4, 4A, 5, 6, 7, 8-六氢-4, 4A-二甲基-6-( 1-甲基亚乙基) -( 4R-顺) -2( 3H) -萘酮、氧化芳樟醇、( E, E) -3, 7, 11, 15-四甲基-1, 6, 10, 14-十六碳四烯-3-醇。该论文对于研究大规模提取柚子皮挥发油具有重大意义，对于挥发油的今后科研工作更具有参考作用。为柚子皮挥发油的提取项目整体规划发展指明了方向。 关键词：柚子皮；挥发油；单因素；成分；气相色谱-质谱 毕业设计（论文）外文摘要 The extraction and content determination of volatile oil from Pomelo peel Abstract: The experiment of extracting the volatile oil from Pomelo peel is conducted to study the factors affecting the extraction of volatile oil with steam distillation using the normal temperature extraction technology. In the experiment, distilled water is used as extraction reagent. The results show that, the extraction duration is 12h, HYPERLINK /Extraction%20time Extraction time is 8h, the ratio of material to water is 1:26, is the best extraction conditions of the experiment. This essay also determines the composition of the volatile oil and its chemical properties are analyzed by GC-MS spectrometry. 62 peaks were isolated from the the volatile oil of artemisia anethifolia, and 31 compounds were identified, accounting for 50% of the ion peak. Their chemical constituents and relative contents were analyzed by GC- MS method. 62components were determined and 31compounds were identified in them,and they were 50. 0% ofthe total volatile oil.The main constituents are ?-pinene, 4, 4A, 5, 6, 7, 8-hexahydro-4, 4A-dimethyl-6 -( 1-methylethylidene) -( 4R-cis) -2( 3H) -na