3种新烟碱类杀虫剂在蜂蜜中HPTLC残留分析方法研究.PDFVIP

安徽农业大学学报, 2018, 45(4): 735-739 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x031 网络出版时间：2018/8/28 9:23:02 [URL] /kcms/detail/34.1162.S1807.062.html 3 种新烟碱类杀虫剂在蜂蜜中 HPTLC 残留分析方法的研究 1 2* 2 梁 睿 ，岳永德 ，汤 锋 (1. 安徽农业大学资源与环境学院，合肥 230036 ；2. 国际竹藤中心国家林业局竹藤科学与技术重点实验室，北京 100102) 摘 要：建立了吡虫啉、噻虫胺和噻虫嗪在蜂蜜中残留的高效薄层色谱（HPTLC ）检测方法，用乙腈机械震荡 提取后涡旋并离心样品，于 C-18 固相萃取柱净化，CAMAG Linomat Ⅳ点样仪点样，二氯甲烷∶乙酸乙酯= 3 ∶1 (V ∶V)混合溶液单向上行展开，于 254 nm 波长进行 HPTLC 检测。结果表明，当 3 种农药在蜂蜜中的添加浓度分别 -1 0.05、0.5 和 1.0 mg·kg 时，添加回收率分别为71.58%～101.25%、72.29%～109.47%和 79.88%～108.92%，相对标准 偏差分别为 9.31%～14.47%、7.79%～12.86%和 9.24%～14.23%，均满足农残分析的基本要求。 关键词：新烟碱类杀虫剂；蜂蜜；高效薄层色谱；农残分析 中图分类号：S481.8 文献标识码：A 文章编号：1672352X (2018)04073505 Study on the residue analysis method of three neonicotinoid insecticides in honey 1 2 2 LIANG Rui , YUE Yongde , TANG Feng (1. School of Resources and Environment, Anhui Agricultural University, Hefei 230036; 2. Key Laboratory of Science and Technology of Bamboo and Rattan, International Centre for Bamboo and Rattan, State Forestry Administration, Beijing 100102) Abstract: A method was developed for analysis of imidacloprid, clothianidin and thiamethoxam residues in honey by high performance thin layer chromatography (HPTLC). Samples were extracted with acetonitrile (accom- panied by mechanical shaking), and cleaned up by C-18 solid-phase extraction column. The extracts were applied as bands to the glass-backed silica gel 60F_(254) HPTLC plates with system of methylene chloride: ethyl acetate = 3:1 (V/V) under unidirectional upward. The plates w