蛋白质合成细胞工厂与微生物代谢资料教程.pptxVIP

蛋白质合成细胞工厂与微生物代谢研究组;蛋白质合成细胞工厂与微生物代谢研究组 Protein production cell factory and microbial metabolism Laboratory;研究组成员介绍;二、蛋白质合成细胞工厂、 酶制剂、医药蛋白;蛋白表达系统平台建立;;;;实例一、高温淀粉酶的表达优化;实例二、蛋白酶表达菌株的分离优化;实例二、蛋白酶表达菌株的分离优化;实例二、蛋白酶表达菌株的分离优化;实例二、蛋白酶表达菌株的分离优化;实例三、异构酶DPE的分泌表达优化;;Media;;三、微生物代谢 维生素B12、氨基酸; 维生素B12组学分析与代谢工程改造;2018/8/22;VB12菌株改造策略;VB12菌株性质及遗传方法建立;VB12菌株全基因组测序与功能途径分析; VB12菌株的代谢途径改造 ;;苏氨酸菌株蛋白组学解析及生理功能鉴定;蛋白组学解析苏氨酸菌株高产机制;高通量快速检测苏氨酸的方法;苏氨酸新转运蛋白的挖掘与鉴定;苯丙氨酸菌株组学解析与工程菌株构建评价;苯丙氨酸工程菌株改造;苯丙氨酸工程菌株改造策略;苯丙氨酸菌株基因组测序分析;苯丙氨酸高产菌株高产机理分析;;理性设计指导菌株改造;异亮氨酸细胞工厂改造;;异亮氨酸细胞工厂构建策略;异亮氨酸细胞工厂构建策略;异亮氨酸细胞工厂构建策略; Song Y?, Nikoloff JM?, Fu G, Chen J, Li Q, Xie N, Zheng P, Sun J,?Zhang D*. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications. PLoS One. 2016 Jul 5;11(7):e0158447. doi: 10.1371/journal. pone.0158447 Jingqi?Chen?,Liuqun?Zhao?,Gang?Fu,Wenjuan?Zhou,Yuanxia?Sun,Ping?Zheng,Jibin?Sun and Dawei?Zhang*. A novel strategy for protein production using non-classical secretion pathway in?Bacillus subtilis. Microbial Cell Factories. 2016,15:69. DOI:?10.1186/s12934-016 -0469-8. Huina Dong, Sha Li, Miaomiao Xia, Ping Zheng*, Dawei Zhang*, Jibin Sun. A Newly Isolated and Identified Vitamin B12 Producing Strain—Sinorhizobium meliloti 320.. Bioprocess and Biosystems Engineering. 2016. DOI：10.1007/s00449-016-1628-3. Jingqi Chen, Yuanming Gai, Gang Fu, Wenjuan Zhou, Dawei Zhang*, Jianping Wen. Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnol Lett. 2014 Dec 17. Huan?Fang,?Huina?Dong,Tao?Cai,?Ping?Zheng,?Haixing?Li,?Dawei?Zhang*?and Jibin?Sun. In?vitro?optimization?of?enzymes?involved?in?precorrin-2 synthesis?using?response?surface?methodology. PloS?One. 2016?accepted. DOI：10.1371/journal.pone.0151149. Jingqi Chen,?Gang Fu,?Yuanming Gai,?Ping Zheng,?Dawei Zhang* and?Jianping Wen. Combinatorial Sec pathway