常见的代谢组学在肿瘤研究中的应用.ppt

* * glutamate dehydrogenase, glutamate oxaloacetate transaminase, glutamine synthetase, glutaminase, glutamate semialdehyde dehydrogenase. 谷氨酸脱氢酶 2.谷氨酸草酰乙酸氨基移转酶 3. 谷氨酰胺合成酶 4.谷氨酰胺酶 5.谷氨酸半醛脱氢酶 草酰乙酸 磷酸烯醇式丙酮酸 谷胱甘肽glutathione aminobutyrate氨基丁酸 proline比咯氨酸 半胱氨酸+谷氨酸→谷胱甘肽 * * * * * * * * * * * * * * Transcription initiation by Pol I requires at least three basal factors, termed Transcription Initiation Factor I (TIF-I) A, TIF-IB/ SL1 and Upstream Binding Factor (UBF) * * * * * * * * * * * Hallmarks of Cancer: The Next Generation * * * * * * * * * * * * 质谱法是通过将样品转化为运动的气态离子并按照质量/电荷比大小进行分离并记录其信息的分析方法. * * * * * * * * * * * * * * SCO2是合成细胞色素C氧化酶（COX）所必需的一个编码铜结合蛋白. P53能通过一个重要靶蛋白SCO2（细胞色素C氧化酶复合物的关键调节亚基）调控有氧呼吸。SCO2基因是合成细胞色素C氧化酶（COX）所必需的一个编码铜结合蛋白的核基因。COX缺乏减少细胞色素C生成，降低了膜电位，减少了ATP生成，从而影响线粒体能量的产生. * * * * * * * 2-1 p53 in tumor cell metabolism: pentose phosphate pathway Figure 2. p53 regulates NADPH levels, lipid accumulation and G6PD activity 2-1 p53 in tumor cell metabolism: pentose phosphate pathway Figure 3 p53 interacts with G6PD and inhibits its activity independently of transcription. 2-1 p53 in tumor cell metabolism: pentose phosphate pathway Figure 4 p53 inhibits the formation of dimeric G6PD holoenzyme. p53 regulation of energy metabolism 2-1 p53 in tumor cell metabolism 2-1 p53 in tumor cell metabolism: glutamine metabolism Biochemistry of glutamine metabolism (main pathways): glutaminase 2-1 p53 in tumor cell metabolism: glutamine metabolism 2-1 p53 in tumor cell metabolism: glutamine metabolism Model for regulation of intracellular ROS levels by GLS2. 2. 2 HIF-1 与肿瘤代谢 2-2 HIF-1 in tumor cell metabolism HIF-1: upstream and downstream of cancer metabolism. Curr Opin Genet Dev. 2010 Feb;20(1):51-6. 2-2 HIF-1 in tumor cell metabolism HIF-1: activating transcription of genes encoding glucose transporters and glycolytic enzyme Expression of genes encoding glucose transporters and glycolytic enzymes. The glycolytic