材料与方法中国植物生理与分子生物学学会.doc

不同基因型大麦苗期耐低氮性状与产量性状的相关性研究 陈志伟，邹磊，陆瑞菊，王亦菲，何婷，杜志钊，张艳敏，黄剑华修回日期：2009.9.21 修回日期：2009.9.21 基金项目：国家科技支撑计划(2006BAD02B04-10)，大麦现代产业技术体系(NYCYTX-029)，上海市农科院青年科技发展基金[农青年科技2007(06)，农青年科技2009(20)]。 第一作者简介：陈志伟(1980- )，男，江苏溧阳人，硕士，研究方向为植物分子育种，E-mail: HYPERLINK mailto:czw1900@ czw1900@,Tel: 021 通讯作者：黄剑华(1953- )，男，上海人，研究员，博士，研究方向为植物细胞工程育种, Tel: 021 E-mail: sw1@。 （上海市农业科学院生物技术研究所，上海 201106；上海市农业遗传育种重点实验室，上海 201106） 摘要：为大麦耐低氮育种提供理论依据，本实验利用溶液培养和大田产量试验相结合的方法，探索了大麦苗期和成熟期各相关性状分别在两种供氮条件下的变异情况。结果表明，苗期氮素生理利用效率、单株吸氮量、地上部干重和苗高在品种间存在极显著差异，籽粒产量在品种间存在显著差异，不同供氮水平对各性状都有极显著的影响。相对性状的基因型变异为：相对地上部干重＞相对分蘖数＞相对单株吸氮量＞相对籽粒产量＞相对氮素利用率＞相对苗高。相关性分析表明低氮胁迫下分蘖数与籽粒产量呈极显著正相关关系，苗期相对氮素生理利用效率、相对苗高和成熟期相对籽粒产量间互呈显著或极显著正相关关系。从各性状的相对值中可以看出大麦品种BI-04表现最好，因而具有较强的耐低氮性，BI-45表现最差，相对比较敏感。 关键词：大麦；耐低氮；氮素生理利用效率；单株吸氮量；分蘖数；籽粒产量 The Study on the Relationship between the Traits for Low-nitrogen Tolerance of Different Barley Genotypes at Seedling Stage and Grain Yield CHEN Zhi-Wei, Zou Lei, LU Rui-Ju, WANG Yi-Fei, HE Ting, DU Zhi-Zhao, Zhang Yan-Min, HUANG Jian-Hua* (Biotech Research Institute, SAAS, Shanghai 201106; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106) Abstract: To provide theoretical basis for low nitrogen breeding, a hydroponic experiment and a field trail were carried out to investigate the index variations for low-N tolerance in Barley under low-N stress and normal-N supply condition, respectively. The result showed that there were significant differences in nitrogen utilization efficiency, nitrogen uptake per plant, shoot dry weight and plant height at seedling stage and differences in grain yield among barley varieties at seedling stage, and there were significant differences in each trait between nitrogen supplies. The relative traits analysis suggested that the relative shoot dry weight, the relative tiller number and the relative nitrogen uptake per plant had the bigger genotype variations. And the correlation analysis indicated that there was a significantly positiv