花生双受精过程及其经历时间-作物学报.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2014, 40(8): 15131519 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.01513 花生双受精过程及其经历时间 * 史春艳 申家恒 李 伟 , 150080 : , , 1 h , 3 h 8 h , 9~13 h , 30 h 2 , , , , 32 h 19 h, , , 13 h , , , 15 h , , : (Arachis hypogaea L.); ; ; ; Double Fertilization and Duration of Phases in Peanut (Arachis hgpogaea L.) * SHI Chun-Yan, SHEN Jia-Heng , and LI Wei College of Life Science and Technology, Harbin Normal University, Harbin 150080, China Abstract: We used paraffin section technology to observe fertilization and duration of phased in fertilization of Arachis hypogaea L. The results indicated that reproductive cell in the pollen came into mitosis tube one hour after bloom, the mitosis lasted about three hours. Sperms released to embryonary sac at about eight hours after flowering. Sperm and egg cell or polar nucleus or sec- ondary nucleus fused from nine to fifteen hours after flowering. Fusion needed about four hours. There were obvious isometrical two nuclei in the zygote at 30 hours after the two nucleole fusion, which came into division stage. Most of zygote had transverse division, and a few longitudinal division. First division needed about 36 hours after bloom, then formed two-cell proembryo and zygote had a dormant period for about 19 hours. After polar nucleus or secondary nucleus fertilization, almost the fusion com- pleted in 13 hours after bloom, and male nucleolus appea