灵芝三萜皂苷GCTL1的体外抗氧化作用-食品科学.PDFVIP

※基础研究 食品科学 2010, Vol. 31, No. 19 49 1 2 崔月花 ，章克昌 (1.扬州大学生物科学与技术学院，江苏 扬州 225009 ； 2.江南大学 教育部工业生物技术重点实验室，江苏 无锡 214122) 采用化学模拟体系研究灵芝三萜皂苷(GCTL1)对超氧阴离子自由基、羟自由基、DPPH 自由基及过氧化氢 自由基的清除能力，测定其还原力和铁螯合力，并与合成的抗氧化剂进行比较。结果表明，GCTL1 具有较好的 抗氧化作用。GCTL1 清除超氧阴离子自由基、羟自由基、DPPH 自由基、过氧化氢自由基的IC50 分别为：(0.247 ± 0.05) 、(0.197 ±0.002) 、(0.09 ±0.02) 、(0.279 ±0.04)mg/mL ；还原力为0.5 、铁螯合力为50% 时GCTL1 质量浓度分 别为(0.425 ±0.01)、(0.180 ±0.04)mg/mL ，并且在一定质量浓度范围内具有剂量依赖效应；GCTL1 的总体抗氧化能 力高于VC 和 2,6 二叔丁基对甲酚(BHT) 。 灵芝；三萜皂苷；抗氧化活性；清除率 Assessment of in vitro Antioxidant Activity of a Triterpenoidal Saponin from Ganoderma lucidum Fruit Bodies 1 2 CUI Yue-hua ，ZHANG Ke-chang (1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China；2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China) Abstract ：To assess the in vitro antioxidant activity of a triterpenoidal saponin from Ganoderma lucidum, named GCTL1, GCTL1 was tested for its abilities to scavenge superoxide anion, hydroxyl, DPPH and hydroxyl peroxide free radicals, reducing power and ferric-chelating capacity and was compared with BHT and vitamin C, synthetic antioxidants. GCTL1 had interesting antioxidant effect. The IC50 values for scavenging superoxide anion, hydroxyl, DPPH and hydroxyl peroxide were (0.247 ± 0.05), (0.197 ± 0.002), (0.09 ± 0.02) mg/mL and (0.279 ± 0.04) mg/mL, respectively, and the concentrations for achieving 0.5 reducing power and 50% ferric-chelating capacity were (0.425 ± 0.01) mg/mL and (0.180 ± 0.04)