α肾上腺素能受体激动对TLR4信号通路影响的-研究.pdfVIP

液离心后上清液中IL一6及IL．18、单核细胞NF．1cBP65蛋白的表达为：Si+NE75 组及空白组比较均明显增加(p0．05)。 结论： 1)Q受体激动剂呈剂量依赖性诱导人外周血单核细胞TLR4信号通路激活， 以759mol／L去甲肾上腺素为最佳剂量； 2)a受体、P38MAPK、PKA、NF．r,BP65介导了o【受体激动剂诱导的TLR4 信号通路激活。 关键词：TLR4Q．肾上腺素能受体激动剂信号通路 Thecrosstalkbetween inducedand Q-adrenoceptoragonist Toll--like 4mediated receptor signalingpathway Master degree Candidate：ZhangXing Supervisor：Prof．QuPeng Medicine Major：Internal Abstract oftheeffectonT0ll—like Objective：Toinvestigation and factorin transductiontheassociated blood duetothe signal peripherymonocytes activationof theinteractionof andTLR4and a··adrenoceptor,toexplore a--adrenoceptor the mechanisminvolved． regulatory Methods：Bloodwerecollectedfrom at samples healthyyoungpeopleperiphery andfreeze-conservedfor／nvitro ex抽ainetheeffectof experiments．(1)T0 Oll mRNA and of a-adrenoceptoragonistnorepinephrineprotein TLR4，P38mRNA， in of blood monocytes andinflammationfactor NF—r,Bp65 periphery andinterleukin-1 serunl．Theblooddonorswere10 8(IL一18)in healthyyoungpeople andcollected32ml venousbloodindividual．Aftertreatment、丽tll periphery blood were toblank NE anticoagulantEDTA，Thesamplesgrouped lamoUL